PolarProtDb

Gene name

NGFR (Tnfrsf16)

Experiment(s)

For this entry:

TNR16_HUMAN (NGFR)

Species	Homo sapiens
Localization	Apical plasma membrane
Reference	Yin X,Kang JH,Andrianifahanana M,Wang Y,Jung MY,Hernandez DM,Leof EB (2017) Basolateral delivery of the type I transforming growth factor beta receptor is mediated by a dominant-acting cytoplasmic motif., Mol Biol Cell, 28, 2701-2711. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Kidney
Cell	MDCK cells (distal tubule)
Notes

For ortholog entries:

TNR16_RAT

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	28	Signal peptide
29	254	Extracellular
255	272	Membrane
273	427	Cytoplasmic

Phosphorylation

Position	Resource
277	9993601
287	41389901
293	7104940
303	10432300
305	41389902
308	41389900
311	34501418
313	7166801
325	34506280
336	454435
425	41389903

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
34	O-GlycoProteomDB
60	Glyconnect
60	Glyconnect
60	Glyconnect	$WURCS=2.0/4,12,11/[Aad21122h-2x_2-6_5NCC/3=O][axxxxm-1x_1-5][axxxxh-1x_1-5_2NCC/3=O][axxxxh-1x_1-5]/1-1-2-3-3-3-3-4-4-4-4-4/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?$
60	Glyconnect	$WURCS=2.0/3,10,9/[axxxxm-1x_1-5][axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-2-2-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?$
60	Glyconnect
60	Glyconnect	$WURCS=2.0/4,11,10/[Aad21122h-2x_2-6_5NCC/3=O][axxxxm-1x_1-5][axxxxh-1x_1-5_2NCC/3=O][axxxxh-1x_1-5]/1-2-3-3-3-3-4-4-4-4-4/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?$
60	N-GlycoSiteAtlas		24495048
60	UniCarbKB		8627329
60	UniProtKB
233	O-GlycoProteomDB
234	O-GlycoProteomDB
238	O-GlycoProteomDB
239	O-GlycoProteomDB

Family and domain data

Database	Domain/Family name
InterPro	DEATH-like_dom_sf
InterPro	Death_domain
InterPro	TNFR/NGFR_Cys_rich_reg
InterPro	TNFR16_TM
InterPro	TNFR_16
InterPro	TNFRSF16_N
Pfam	Death
Pfam	TNFR_16_TM
Pfam	TNFR_c6

Interactions

With (links to EBI)	#Exp	PubMed links
CD80_HUMAN	3	21982860
A4_HUMAN	2	11756426

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.