Evidence Levels
(classification of experimental methods)
| Experimental procedures | Experimental evidence type |
|---|---|
|
Immunfluorescence (confocal microscopy) Immunogold labeling (electron microscopy) | Antibody staining |
| Confocal microscopy of GFP-tagged proteins | Fluorescent labeling |
|
Isolation of of brush border and basal membranes Side-specific release of membrane proteins with enzyme Side-specific biotinylation of proteins followed by purification and/or western blots | Membrane preparation |
|
Western blot of proteins in apical or basolateral media Immunoprecipitation of secreted proteins in either media | Selective protein analysis |
|
Protease, nuclease or other enzymatic assay in situ with
chromogenic substrate Uptake or transport assays of specific substrates | Functional assay |
| Mass-spectrometry of side-specifically labelled endogenous proteins | Large-scale proteomics |
| Comparison of specifically designed, localization-defective mutants with WT proteins | Sub-protein manipulation |
| Co-expression or co-purification experiments Viral infection susceptibility assays | Other (specified in notes) |