PolarProtDb

Gene name

SLC9A1 (NHE1,APNH1)

Experiment(s)

For this entry:

SL9A1_MOUSE (SLC9A1)

Species	Mus musculus
Localization	Basolateral plasma membrane
Reference	Nejsum LN,Praetorius J,Nielsen S (2005) NKCC1 and NHE1 are abundantly expressed in the basolateral plasma membrane of secretory coil cells in rat, mouse, and human sweat glands., Am J Physiol Cell Physiol, 289, C333-40. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Sweat glands
Cell	Secretory cells
Notes	Immunogold labelling under electron microscope

For ortholog entries:

SL9A1_RAT

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	15	Cytoplasmic
16	33	Membrane
34	107	Extracellular
108	126	Membrane
127	135	Cytoplasmic
136	153	Membrane
154	163	Extracellular
164	181	Membrane
182	191	Cytoplasmic
192	215	Membrane
216	231	Extracellular
232	250	Membrane
251	258	Cytoplasmic
259	277	Membrane
278	296	Extracellular
297	322	Membrane
323	331	Cytoplasmic
332	348	Membrane
349	350	Extracellular
351	367	Membrane
368	388	Cytoplasmic
389	407	Membrane
408	416	Extracellular
417	438	Membrane
439	454	Cytoplasmic
455	473	Membrane
474	482	Extracellular
483	503	Membrane
504	820	Cytoplasmic

Phosphorylation

Position	Resource
581	3719831
603	468290
606	468291
607	468294
609	468292
628	36698474
641	40512552
687	474351
689	2023305
697	468293
699	1983421
701	3181646
707	448347
712	15404919
722	4059100
727	2509103
730	2509107
733	3181649
755	4729336
771	18262072
776	475074
779	4729342
784	3914103
787	14576821
788	3181652
790	1983418
792	460912
793	460913
801	460570

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
76	Glyconnect	$WURCS=2.0/2,9,8/[axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-1-1-1-1-2-2-2/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?$	30899004

Family and domain data

Database	Domain/Family name
InterPro	Cation/H_exchanger
InterPro	Cation/H_exchanger_CPA1
InterPro	Na/H_exchanger_1
InterPro	NaH_exchanger
InterPro	NHE_CaM-bd
Pfam	Na_H_Exchanger
Pfam	NEXCaM_BD