PolarProtDb

Gene name

Slc12a7 (Kcc4)

Experiment(s)

For this entry:

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	118	Cytoplasmic
119	139	Membrane
140	153	Extracellular
154	175	Membrane
176	198	Cytoplasmic
199	220	Membrane
221	250	Extracellular
251	270	Membrane
271	275	Cytoplasmic
276	296	Membrane
297	415	Extracellular
416	436	Membrane
437	455	Cytoplasmic
456	477	Membrane
478	498	Extracellular
499	520	Membrane
521	556	Cytoplasmic
557	574	Membrane
575	579	Extracellular
580	600	Membrane
601	616	Cytoplasmic
617	634	Membrane
635	636	Extracellular
637	654	Membrane
655	849	Cytoplasmic
850	865	Membrane
866	1083	Extracellular

Eukaryotic Linear Motif (ELM)

Start	End	Sequence	Motif
108	111	SRRR	TRG_ER_diArg_1

Phosphorylation

Position	Resource
6	31020410
25	31872525
30	477871
40	3217701
50	468760
62	3217704
78	31880737
92	474153
93	34605965
96	31884186
108	23117499
669	17544764
715	10790551
716	10790555
735	55380663
926	12738728
968	17551911
973	4786481
980	4786486
982	4786491
991	460288
996	5207784
997	4836905
1006	34365843
1012	34365846

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
312	Glyconnect	$WURCS=2.0/2,11,10/[axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-2-2-2-2-2-2-2-2-2/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?$	29741879
312	N-GlycoSiteAtlas		24190977
1029	N-GlycoSiteAtlas		24741114

Family and domain data

Database	Domain/Family name
InterPro	AA-permease/SLC12A_dom
InterPro	KCC4
InterPro	KCL_cotranspt
InterPro	SLC12_C
InterPro	SLC12A_fam
Pfam	AA_permease
Pfam	SLC12

Interactions

With (links to EBI)	#Exp	PubMed links
NTCP_HUMAN	3	32296183
TGON2_HUMAN	3	29568061
TMM17_HUMAN	2	26638075

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.