Gene name
SLC12A2 (Nkcc1)
Experiment(s)
For this entry:
Q9QX10_RAT (SLC12A2)
| Species | Rattus norvegicus |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
O'Donnell ME,Tran L,Lam TI,Liu XB,Anderson SE (2004) Bumetanide inhibition of the blood-brain barrier Na-K-Cl cotransporter reduces edema formation in the rat middle cerebral artery occlusion model of stroke., J Cereb Blood Flow Metab, 24, 1046-56. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Vessels |
| Cell | Brain capillary endothelial cells |
| Notes |
Q9QX10_RAT (SLC12A2)
| Species | Rattus norvegicus |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Nejsum LN,Praetorius J,Nielsen S (2005) NKCC1 and NHE1 are abundantly expressed in the basolateral plasma membrane of secretory coil cells in rat, mouse, and human sweat glands., Am J Physiol Cell Physiol, 289, C333-40. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Sweat glands |
| Cell | Secretory cells |
| Notes |
Q9QX10_RAT (SLC12A2)
| Species | Rattus norvegicus |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Jakab RL,Collaco AM,Ameen NA (2011) Physiological relevance of cell-specific distribution patterns of CFTR, NKCC1, NBCe1, and NHE3 along the crypt-villus axis in the intestine., Am J Physiol Gastrointest Liver Physiol, 300, G82-98. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Small intestine |
| Cell | Enterocytes |
| Notes | "Mostly located in endosomes |
Q9QX10_RAT (SLC12A2)
| Species | Rattus norvegicus |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Jakab RL,Collaco AM,Ameen NA (2011) Physiological relevance of cell-specific distribution patterns of CFTR, NKCC1, NBCe1, and NHE3 along the crypt-villus axis in the intestine., Am J Physiol Gastrointest Liver Physiol, 300, G82-98. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Small intestine |
| Cell | Goblet cells |
| Notes |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 281 | Cytoplasmic |
| 282 | 303 | Membrane |
| 304 | 305 | Extracellular |
| 306 | 333 | Membrane |
| 334 | 354 | Cytoplasmic |
| 355 | 376 | Membrane |
| 377 | 399 | Extracellular |
| 400 | 419 | Membrane |
| 420 | 426 | Cytoplasmic |
| 427 | 446 | Membrane |
| 447 | 476 | Extracellular |
| 477 | 496 | Membrane |
| 497 | 512 | Cytoplasmic |
| 513 | 534 | Membrane |
| 535 | 592 | Extracellular |
| 593 | 614 | Membrane |
| 615 | 644 | Cytoplasmic |
| 645 | 666 | Membrane |
| 667 | 668 | Extracellular |
| 669 | 690 | Membrane |
| 691 | 706 | Cytoplasmic |
| 707 | 734 | Membrane |
| 735 | 1203 | Extracellular |
Phosphorylation
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | AA-permease/SLC12A_dom |
| InterPro | AA_permease_N |
| InterPro | NKCC1 |
| InterPro | SLC12_C |
| InterPro | SLC12A1/SLC12A2 |
| InterPro | SLC12A_fam |
| Pfam | AA_permease |
| Pfam | AA_permease_N |
| Pfam | SLC12 |