Gene name
Slc34a2 (Npt2b)
Experiment(s)
For this entry:
NPT2B_HUMAN (Slc34a2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Giral H,Cranston D,Lanzano L,Caldas Y,Sutherland E,Rachelson J,Dobrinskikh E,Weinman EJ,Doctor RB,Gratton E,Levi M (2012) NHE3 regulatory factor 1 (NHERF1) modulates intestinal sodium-dependent phosphate transporter (NaPi-2b) expression in apical microvilli., J Biol Chem, 287, 35047-56. [PubMed] [DOI] |
| Evidence level | Fluorescent tagging |
| Organ | Large intestine |
| Cell | CACO-2 cells (enterocytes) |
| Notes | Apical localization depended on the C-terminal PDZ motif and its interaction with NHERF |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 100 | Cytoplasmic |
| 101 | 122 | Membrane |
| 123 | 138 | Extracellular |
| 139 | 157 | Membrane |
| 158 | 220 | Cytoplasmic |
| 221 | 239 | Membrane |
| 240 | 361 | Extracellular |
| 362 | 383 | Membrane |
| 384 | 408 | Cytoplasmic |
| 409 | 430 | Membrane |
| 431 | 432 | Extracellular |
| 433 | 451 | Membrane |
| 452 | 456 | Cytoplasmic |
| 457 | 475 | Membrane |
| 476 | 486 | Extracellular |
| 487 | 507 | Membrane |
| 508 | 526 | Cytoplasmic |
| 527 | 548 | Membrane |
| 549 | 553 | Extracellular |
| 554 | 575 | Membrane |
| 576 | 690 | Cytoplasmic |
Motifs gathered by PolarProtDb Team
| Start | End | Localisation | Type | PubMed |
|---|---|---|---|---|
| 685 | 690 | Apical plasma membrane | PDZ-ligand |
Giral H,Cranston D,Lanzano L,Caldas Y,Sutherland E,Rachelson J,Dobrinskikh E,Weinman EJ,Doctor RB,Gratton E,Levi M (2012) NHE3 regulatory factor 1 (NHERF1) modulates intestinal sodium-dependent phosphate transporter (NaPi-2b) expression in apical microvilli., J Biol Chem, 287, 35047-56. [PubMed][DOI] |
Phosphorylation
Glycosylation
| Position | Site resource | Glycan resource | PubMed link(s) |
|---|---|---|---|
| 136 | N-GlycoSiteAtlas | ||
| 295 | N-GlycoSiteAtlas | 24164404 | |
| 308 | N-GlycoSiteAtlas | 26571101 | |
| 313 | N-GlycoSiteAtlas | 26571101 | |
| 321 | N-GlycoSiteAtlas | 26571101 | |
| 340 | N-GlycoSiteAtlas | 24164404 , 26571101 , 26571101 |
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | Na/Pi_transpt |
| InterPro | Na/Pi_transpt_2B |
| Pfam | Na_Pi_cotrans |
Interactions
| With (links to EBI) | #Exp | PubMed links |
|---|---|---|
| S35A1_HUMAN | 3 | 32296183 |
Protein can be found in apical membrane,
Protein can be found in basal membrane,
Protein can be found both membranes,
Localisation is unknown.