Gene name
ABCC2 (Cmoat,CMOAT1,Cmrp,Mrp2)
Experiment(s)
For this entry:
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Wojtal KA,de Vries E,Hoekstra D,van Ijzendoorn SC (2006) Efficient trafficking of MDR1/P-glycoprotein to apical canalicular plasma membranes in HepG2 cells requires PKA-RIIalpha anchoring and glucosylceramide., Mol Biol Cell, 17, 3638-50. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Liver |
| Cell | HEPG2 cells (hepatocytes) |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Emi Y,Yasuda Y,Sakaguchi M (2012) A cis-acting five-amino-acid motif controls targeting of ABCC2 to the apical plasma membrane domain., J Cell Sci, 125, 3133-43. [PubMed] [DOI] |
| Evidence level | Antibody staining + sub-protein manipulation |
| Organ | Liver |
| Cell | HEPG2 cells (hepatocytes) |
| Notes | A dileucine-type apical targeting signal was identified: SQDALV (likely phosphorylated) |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Mooij MG,de Koning BE,Lindenbergh-Kortleve DJ,Simons-Oosterhuis Y,van Groen BD,Tibboel D,Samsom JN,de Wildt SN (2016) Human Intestinal PEPT1 Transporter Expression and Localization in Preterm and Term Infants., Drug Metab Dispos, 44, 1014-9. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Large intestine |
| Cell | Enterocytes |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Mooij MG,de Koning BE,Lindenbergh-Kortleve DJ,Simons-Oosterhuis Y,van Groen BD,Tibboel D,Samsom JN,de Wildt SN (2016) Human Intestinal PEPT1 Transporter Expression and Localization in Preterm and Term Infants., Drug Metab Dispos, 44, 1014-9. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Large intestine |
| Cell | Goblet cells |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Nies AT,Koepsell H,Winter S,Burk O,Klein K,Kerb R,Zanger UM,Keppler D,Schwab M,Schaeffeler E (2009) Expression of organic cation transporters OCT1 (SLC22A1) and OCT3 (SLC22A3) is affected by genetic factors and cholestasis in human liver., Hepatology, 50, 1227-40. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Liver |
| Cell | Hepatocytes |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Paulusma CC,Kool M,Bosma PJ,Scheffer GL,ter Borg F,Scheper RJ,Tytgat GN,Borst P,Baas F,Oude Elferink RP (1997) A mutation in the human canalicular multispecific organic anion transporter gene causes the Dubin-Johnson syndrome., Hepatology, 25, 1539-42. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Liver |
| Cell | Hepatocytes |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Evers R,Kool M,van Deemter L,Janssen H,Calafat J,Oomen LC,Paulusma CC,Oude Elferink RP,Baas F,Schinkel AH,Borst P (1998) Drug export activity of the human canalicular multispecific organic anion transporter in polarized kidney MDCK cells expressing cMOAT (MRP2) cDNA., J Clin Invest, 101, 1310-9. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Kidney |
| Cell | MDCK cells (distal tubule) |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Schaub TP,Kartenbeck J,König J,Spring H,Dörsam J,Staehler G,Störkel S,Thon WF,Keppler D (1999) Expression of the MRP2 gene-encoded conjugate export pump in human kidney proximal tubules and in renal cell carcinoma., J Am Soc Nephrol, 10, 1159-69. [PubMed] |
| Evidence level | Antibody staining |
| Organ | Kidney |
| Cell | Proximal tubules |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
St-Pierre MV,Serrano MA,Macias RI,Dubs U,Hoechli M,Lauper U,Meier PJ,Marin JJ (2000) Expression of members of the multidrug resistance protein family in human term placenta., Am J Physiol Regul Integr Comp Physiol, 279, R1495-503. [PubMed] [DOI] |
| Evidence level | Membrane preparation |
| Organ | Placenta |
| Cell | Trophoblast cells |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Fromm MF,Kauffmann HM,Fritz P,Burk O,Kroemer HK,Warzok RW,Eichelbaum M,Siegmund W,Schrenk D (2000) The effect of rifampin treatment on intestinal expression of human MRP transporters., Am J Pathol, 157, 1575-80. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Small intestine |
| Cell | Enterocytes |
| Notes | Expression is strongly increased by rifampin |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Rost D,König J,Weiss G,Klar E,Stremmel W,Keppler D (2001) Expression and localization of the multidrug resistance proteins MRP2 and MRP3 in human gallbladder epithelia., Gastroenterology, 121, 1203-8. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Gall bladder |
| Cell | Epithelial cells |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Keiser M,Kaltheuner L,Wildberg C,Müller J,Grube M,Partecke LI,Heidecke CD,Oswald S (2017) The Organic Anion-Transporting Peptide 2B1 Is Localized in the Basolateral Membrane of the Human Jejunum and Caco-2 Monolayers., J Pharm Sci, 106, 2657-2663. [PubMed] [DOI] |
| Evidence level | Membrane preparation |
| Organ | Small intestine |
| Cell | Enterocytes |
| Notes | Membranes were analyzed by targeted mass spectrometry instead of western blotting |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Jansen PL,Strautnieks SS,Jacquemin E,Hadchouel M,Sokal EM,Hooiveld GJ,Koning JH,De Jager-Krikken A,Kuipers F,Stellaard F,Bijleveld CM,Gouw A,Van Goor H,Thompson RJ,Müller M (1999) Hepatocanalicular bile salt export pump deficiency in patients with progressive familial intrahepatic cholestasis., Gastroenterology, 117, 1370-9. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Liver |
| Cell | Hepatocytes |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Rius M,Nies AT,Hummel-Eisenbeiss J,Jedlitschky G,Keppler D (2003) Cotransport of reduced glutathione with bile salts by MRP4 (ABCC4) localized to the basolateral hepatocyte membrane., Hepatology, 38, 374-84. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Liver |
| Cell | Hepatocytes |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Meyer Zu Schwabedissen HE,Grube M,Heydrich B,Linnemann K,Fusch C,Kroemer HK,Jedlitschky G (2005) Expression, localization, and function of MRP5 (ABCC5), a transporter for cyclic nucleotides, in human placenta and cultured human trophoblasts: effects of gestational age and cellular differentiation., Am J Pathol, 166, 39-48. [PubMed] [DOI] |
| Evidence level | Membrane preparation |
| Organ | Placenta |
| Cell | Trophoblast cells |
| Notes |
MRP2_HUMAN (ABCC2)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Azzaroli F,Mennone A,Feletti V,Simoni P,Baglivo E,Montagnani M,Rizzo N,Pelusi G,DE Aloysio D,Lodato F,Festi D,Colecchia A,Roda E,Boyer JL,Mazzella G (2007) Clinical trial: modulation of human placental multidrug resistance proteins in cholestasis of pregnancy by ursodeoxycholic acid., Aliment Pharmacol Ther, 26, 1139-46. [PubMed] [DOI] |
| Evidence level | Membrane preparation |
| Organ | Placenta |
| Cell | Trophoblast cells |
| Notes |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 30 | Extracellular |
| 31 | 51 | Membrane |
| 52 | 66 | Cytoplasmic |
| 67 | 87 | Membrane |
| 88 | 97 | Extracellular |
| 98 | 119 | Membrane |
| 120 | 129 | Cytoplasmic |
| 130 | 151 | Membrane |
| 152 | 162 | Extracellular |
| 163 | 184 | Membrane |
| 185 | 317 | Cytoplasmic |
| 318 | 338 | Membrane |
| 339 | 357 | Extracellular |
| 358 | 378 | Membrane |
| 379 | 435 | Cytoplasmic |
| 436 | 456 | Membrane |
| 457 | 460 | Extracellular |
| 461 | 481 | Membrane |
| 482 | 544 | Cytoplasmic |
| 545 | 566 | Membrane |
| 567 | 578 | Extracellular |
| 579 | 600 | Membrane |
| 601 | 972 | Cytoplasmic |
| 973 | 994 | Membrane |
| 995 | 1024 | Extracellular |
| 1025 | 1046 | Membrane |
| 1047 | 1097 | Cytoplasmic |
| 1098 | 1118 | Membrane |
| 1119 | 1120 | Extracellular |
| 1121 | 1141 | Membrane |
| 1142 | 1205 | Cytoplasmic |
| 1206 | 1227 | Membrane |
| 1228 | 1236 | Extracellular |
| 1237 | 1256 | Membrane |
| 1257 | 1545 | Cytoplasmic |
Motifs gathered by PolarProtDb Team
Phosphorylation
| Position | Resource |
|---|---|
| 60 | 56665200 |
| 65 | 50772399 |
| 237 | 23078372 |
| 281 | 5357002 |
| 283 | 3208476 |
| 602 | 56568021 |
| 609 | 56568025 |
| 616 | 10940995 |
| 723 | 18006290 |
| 878 | 3208479 |
| 885 | 34500202 |
| 900 | 3208482 |
| 908 | 29574312 |
| 919 | 50772395 |
| 926 | 29596571 |
| 930 | 3883023 |
| 938 | 14567721 |
| 1153 | 27573000 |
| 1296 | 34506314 |
| 1326 | 34504618 |
| 1438 | 34498101 |
| 1501 | 23078369 |
Glycosylation
| Position | Site resource | Glycan resource | PubMed link(s) |
|---|---|---|---|
| 1011 | N-GlycoSiteAtlas | 24495048 | |
| 1011 | PolarProtDb | 31844046 |
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | AAA+_ATPase |
| InterPro | ABC1_TM_dom |
| InterPro | ABC1_TM_sf |
| InterPro | ABC_transporter-like |
| InterPro | ABC_transporter_CS |
| InterPro | ABCC2 |
| InterPro | Multidrug-R_assoc |
| InterPro | P-loop_NTPase |
| Pfam | ABC_membrane |
| Pfam | ABC_tran |
Interactions
| With (links to EBI) | #Exp | PubMed links |
|---|---|---|
| NFKB1_HUMAN | 3 | 21988832 |
Protein can be found in apical membrane,
Protein can be found in basal membrane,
Protein can be found both membranes,
Localisation is unknown.