Gene name

Abcb1 (Mdr1,Pgy1,Mdr1b)

Experiment(s)

For this entry:
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Florea BI,van der Sandt IC,Schrier SM,Kooiman K,Deryckere K,de Boer AG,Junginger HE,Borchard G (2001) Evidence of P-glycoprotein mediated apical to basolateral transport of flunisolide in human broncho-tracheal epithelial cells (Calu-3)., Br J Pharmacol, 134, 1555-63.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Kidney
Cell LLC-PK1 cells (proximal tubule)
Notes Functional transport assays were also used for validation
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Wojtal KA,de Vries E,Hoekstra D,van Ijzendoorn SC (2006) Efficient trafficking of MDR1/P-glycoprotein to apical canalicular plasma membranes in HepG2 cells requires PKA-RIIalpha anchoring and glucosylceramide., Mol Biol Cell, 17, 3638-50.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Liver
Cell HEPG2 cells (hepatocytes)
Notes Localization was dependent on the proper AKAP proteins and glycolipids
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Basolateral plasma membrane
Reference Florea BI,van der Sandt IC,Schrier SM,Kooiman K,Deryckere K,de Boer AG,Junginger HE,Borchard G (2001) Evidence of P-glycoprotein mediated apical to basolateral transport of flunisolide in human broncho-tracheal epithelial cells (Calu-3)., Br J Pharmacol, 134, 1555-63.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Lung
Cell CALU-3 cells (airway epithel)
Notes Functional transport assays were also used for validation
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Non polar membrane
Reference Mooij MG,de Koning BE,Lindenbergh-Kortleve DJ,Simons-Oosterhuis Y,van Groen BD,Tibboel D,Samsom JN,de Wildt SN (2016) Human Intestinal PEPT1 Transporter Expression and Localization in Preterm and Term Infants., Drug Metab Dispos, 44, 1014-9.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Large intestine
Cell Enterocytes
Notes Clearly Non polar in both infant and adult samples.
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Non polar membrane
Reference Mooij MG,de Koning BE,Lindenbergh-Kortleve DJ,Simons-Oosterhuis Y,van Groen BD,Tibboel D,Samsom JN,de Wildt SN (2016) Human Intestinal PEPT1 Transporter Expression and Localization in Preterm and Term Infants., Drug Metab Dispos, 44, 1014-9.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Large intestine
Cell Goblet cells
Notes
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Nies AT,Herrmann E,Brom M,Keppler D (2008) Vectorial transport of the plant alkaloid berberine by double-transfected cells expressing the human organic cation transporter 1 (OCT1, SLC22A1) and the efflux pump MDR1 P-glycoprotein (ABCB1)., Naunyn Schmiedebergs Arch Pharmacol, 376, 449-61.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Kidney
Cell MDCK cells (distal tubule)
Notes
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Thiebaut F,Tsuruo T,Hamada H,Gottesman MM,Pastan I,Willingham MC (1987) Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues., Proc Natl Acad Sci U S A, 84, 7735-8.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Liver
Cell Hepatocytes
Notes
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Thiebaut F,Tsuruo T,Hamada H,Gottesman MM,Pastan I,Willingham MC (1987) Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues., Proc Natl Acad Sci U S A, 84, 7735-8.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Pancreas
Cell Ductal cells
Notes
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Thiebaut F,Tsuruo T,Hamada H,Gottesman MM,Pastan I,Willingham MC (1987) Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues., Proc Natl Acad Sci U S A, 84, 7735-8.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Large intestine
Cell Enterocytes
Notes
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Thiebaut F,Tsuruo T,Hamada H,Gottesman MM,Pastan I,Willingham MC (1987) Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues., Proc Natl Acad Sci U S A, 84, 7735-8.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Small intestine
Cell Enterocytes
Notes
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Thiebaut F,Tsuruo T,Hamada H,Gottesman MM,Pastan I,Willingham MC (1987) Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues., Proc Natl Acad Sci U S A, 84, 7735-8.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Kidney
Cell Proximal tubules
Notes
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Beaulieu E,Demeule M,Ghitescu L,Béliveau R (1997) P-glycoprotein is strongly expressed in the luminal membranes of the endothelium of blood vessels in the brain., Biochem J, 326 ( Pt 2), 539-44.
[PubMed] [DOI]
Evidence level Membrane preparation
Organ Vessels
Cell Brain capillary endothelial cells
Notes
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Kozłowska-Rup D,Czekaj P,Plewka D,Sikora J (2014) Immunolocalization of ABC drug transporters in human placenta from normal and gestational diabetic pregnancies., Ginekol Pol, 85, 410-9.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Placenta
Cell Trophoblast cells
Notes
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Non polar membrane
Reference Keiser M,Kaltheuner L,Wildberg C,Müller J,Grube M,Partecke LI,Heidecke CD,Oswald S (2017) The Organic Anion-Transporting Peptide 2B1 Is Localized in the Basolateral Membrane of the Human Jejunum and Caco-2 Monolayers., J Pharm Sci, 106, 2657-2663.
[PubMed] [DOI]
Evidence level Membrane preparation
Organ Small intestine
Cell Enterocytes
Notes Membranes were analyzed by targeted mass spectrometry instead of western blotting
MDR1_HUMAN (Abcb1)
Species Homo sapiens
Localization Apical plasma membrane
Reference Nagashige M,Ushigome F,Koyabu N,Hirata K,Kawabuchi M,Hirakawa T,Satoh S,Tsukimori K,Nakano H,Uchiumi T,Kuwano M,Ohtani H,Sawada Y (2003) Basal membrane localization of MRP1 in human placental trophoblast., Placenta, 24, 951-8.
[PubMed] [DOI]
Evidence level Antibody staining
Organ Placenta
Cell Trophoblast cells
Notes
For ortholog entries:
MDR1_RAT
MDR1_RAT
MDR1_RAT
MDR1_RAT

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
Start End Localisation
1 45 Cytoplasmic
46 69 Membrane
70 118 Extracellular
119 139 Membrane
140 186 Cytoplasmic
187 208 Membrane
209 215 Extracellular
216 236 Membrane
237 291 Cytoplasmic
292 313 Membrane
314 333 Extracellular
334 355 Membrane
356 710 Cytoplasmic
711 732 Membrane
733 756 Extracellular
757 778 Membrane
779 830 Cytoplasmic
831 851 Membrane
852 858 Extracellular
859 879 Membrane
880 933 Cytoplasmic
934 955 Membrane
956 975 Extracellular
976 996 Membrane
997 1280 Cytoplasmic
Phosphorylation
Position Resource
23 18006278
263 23078303
422 23078306
496 25279253
522 23078309
661 456983
667 455363
671 455365
675 34499801
683 455364
684 23078312
696 23078300
1015 5831385
1023 5831391
1030 5831397
1087 3665346
1267 34362666
1269 34362669
Glycosylation
Position Site resource Glycan resource PubMed link(s)
91 PDB WURCS=2.0/1,1,0/[a2122h-1b_1-5_2*NCC/3=O]/1/ 30765569
91 N-GlycoSiteAtlas 24495048
91 PolarProtDb 31844046
91 UniPep
94 PDB WURCS=2.0/1,1,0/[a2122h-1b_1-5_2*NCC/3=O]/1/ 30765569
94 N-GlycoSiteAtlas 24495048
94 PolarProtDb 31844046
94 UniPep
99 PDB WURCS=2.0/1,1,0/[a2122h-1b_1-5_2*NCC/3=O]/1/ 30765569
99 N-GlycoSiteAtlas 24467213 , 24495048
99 PolarProtDb 31844046

Family and domain data

Database Domain/Family name
InterPro AAA+_ATPase
InterPro ABC1_TM_dom
InterPro ABC1_TM_sf
InterPro ABC_transporter-like
InterPro ABC_transporter_CS
InterPro MDR1
InterPro P-loop_NTPase
InterPro Type_I_exporter
Pfam ABC_membrane
Pfam ABC_tran

Interactions

With (links to EBI) #Exp PubMed links
RING2_HUMAN 2 17088979
Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.

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