Gene name
Slc1a1 (Eaac1,Eaat3,ECO:0000303|PubMed:9011753})
Experiment(s)
For this entry:
EAA3_HUMAN (Slc1a1)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Cheng C,Glover G,Banker G,Amara SG (2002) A novel sorting motif in the glutamate transporter excitatory amino acid transporter 3 directs its targeting in Madin-Darby canine kidney cells and hippocampal neurons., J Neurosci, 22, 10643-52. [PubMed] |
| Evidence level | Fluorescent tagging |
| Organ | Kidney |
| Cell | MDCK cells (distal tubule) |
| Notes | The source of plasmids is unclear. An unusal apical sorting signal has been identified (KSYVNGGFAVD) |
EAA3_HUMAN (Slc1a1)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Bailey CG,Ryan RM,Thoeng AD,Ng C,King K,Vanslambrouck JM,Auray-Blais C,Vandenberg RJ,Bröer S,Rasko JE (2011) Loss-of-function mutations in the glutamate transporter SLC1A1 cause human dicarboxylic aminoaciduria., J Clin Invest, 121, 446-53. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Kidney |
| Cell | Proximal tubules |
| Notes |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 21 | Cytoplasmic |
| 22 | 37 | Membrane |
| 38 | 50 | Extracellular |
| 51 | 72 | Membrane |
| 73 | 94 | Cytoplasmic |
| 95 | 116 | Membrane |
| 117 | 191 | Extracellular |
| 192 | 219 | Membrane |
| 220 | 254 | Cytoplasmic |
| 255 | 270 | Membrane |
| 271 | 283 | Extracellular |
| 284 | 305 | Membrane |
| 306 | 362 | Cytoplasmic |
| 363 | 378 | Membrane |
| 379 | 394 | Extracellular |
| 395 | 410 | Membrane |
| 411 | 412 | Cytoplasmic |
| 413 | 428 | Membrane |
| 429 | 440 | Extracellular |
| 441 | 456 | Membrane |
| 457 | 524 | Cytoplasmic |
Motifs gathered by PolarProtDb Team
| Start | End | Localisation | Type | PubMed |
|---|---|---|---|---|
| 501 | 511 | Apical plasma membrane | Unknown |
Cheng C,Glover G,Banker G,Amara SG (2002) A novel sorting motif in the glutamate transporter excitatory amino acid transporter 3 directs its targeting in Madin-Darby canine kidney cells and hippocampal neurons., J Neurosci, 22, 10643-52. [PubMed] |
Phosphorylation
Glycosylation
![WURCS=2.0/2,2,1/[uxxxxh_2*NCC/3=O][uxxxxh]/1-2/a?|b?}-{a?|b?](data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAALIAAACGCAIAAAD2A0eEAAAD4ElEQVR42u3cIU9qbxzAcWci+AIMBgKBYCT4AggEAsFIckSCgUgkGghG3a4b0WjAzY1gMBAMBKLBF0AgGAz3/9vY3P0PPByPeu89536+ielzmNvz4XmeA8ydn9JaO2nH/dJ7v808/r2RO2u9/Xx95MZLTPA3slif8q3T/KHx7w1LmPuUvyLju1iknK2EYRun6qMsEthh8dexSHgFJ88oFsVhsb5nJ0/zxjNHBhZpdiVniz+8Wmyc2jSTmvIV/0kWVos/zyLNqzPl+K2XY5GzO5HklWDr+Aws1rc2LP669y02zkf68dmWkI2XOFv8VhbCQlhIWAgLYSEshIWwEBbCQlgIC2EhLISFhIWwEBb651j4nmYBWUyn036/X6/XV1N7eHjYaDQGg8F8Pk9v4u2x+cs9i8lkcnR0VKlUgsV4PA4HT09Ps9ns5ubm9PT04OAgfDw+Pm5lsfGx8sfi9fW11+uVy+XRaJQw5vz8fH9//+zs7KPLhvLHIua71WrFSrBYLLYOfn5+rtVq3W6XiYKziDluNpuBI+X45XIZMpLXDCbyzSLODXGYiJn+0FWxZsRusvWcoVyyiBUibjRCRoZr45wR+45NpIAs4nYjbj0yk4p7k/fuWrHIMYtOpzMcDjNffnJyMhgMzFDRWFSr1dlslvnyy8vLhH1EeWWxu7v70cPmr93e3sbRxAwVjcUnt/+Hh4c4XpihorEolUovLy+ZL7+7u4ttyAwVjUWlUkn5AdjGrq6u6vW6GSoai3a7fXFxkfnybrfb7/fNUNFYXF9ff+blHovNdDrdeGTxvkWOWcTBolwu39/fZ7h2NBptfCvM9y1yzyL68eNHrVZL/znZqsViEZ4mk0ny3Q0WeWURHR8fdzqd9OPDUKPR6PV6W+9+scgxi+VyGdtByEizZsTgZrPZarWSBzORexaryY41I3aT5HPG6lP4uAHZCoiJIrB4O2fEiSHuTeKudT6fr97pCjGz2Ww4HMaKkvlTeOWYxereJO5a2+12rAqlUile9Ht7e9VqNbaY8Xic/mRqEykUiy/7o7HAQlgIC2EhLISFsBAWwkLCQlgIC2EhLISFsBAWwkJYCAthISyEhYSFsBAWwkJY/O8v9r+zcs1i5yva+Jxvj81fLll8+TP431lYJD2DfQQLJrBIx8LMYSEsbCJYYIGFTQQLLLDAAgsssMACCyyEhQrEwvctsPB9CyzSsbDFYOH7FlikfgYmsHCHgoXp/xdYfPk3v20iuWfx3eBMHhbCQlgIC2EhLISFsBAWEhbCQlgIC2EhLISFsBAWwkJYCAthISwkLISFsBAWwkK/r/8AbGf8Sd8qlxgAAAAASUVORK5CYII=)
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | Na-dicarboxylate_symporter |
| InterPro | Na-dicarboxylate_symporter_CS |
| InterPro | Na:dicarbo_symporter_sf |
| Pfam | SDF |
Interactions
| With (links to EBI) | #Exp | PubMed links |
|---|---|---|
| RNF5_HUMAN | 6 | 25416956 , 32296183 |
| LRAD1_HUMAN | 4 | 25416956 , 31515488 |
| PAGE1_HUMAN | 3 | 32296183 |
| T179B_HUMAN | 3 | 32296183 |
| ERGI3_HUMAN | 3 | 32296183 |
| SYNPR_HUMAN | 3 | 32296183 |
| F209A_HUMAN | 3 | 32296183 |
| S35A1_HUMAN | 3 | 32296183 |
| FXYD3_HUMAN | 3 | 32296183 |
Protein can be found in apical membrane,
Protein can be found in basal membrane,
Protein can be found both membranes,
Localisation is unknown.