PolarProtDb

Gene name

Anpep (APN,CD13,PEPN,Lap-1,Lap1)

Experiment(s)

For this entry:

AMPN_PIG (Anpep)

Species	Sus scrofa
Localization	Apical plasma membrane
Reference	Vogel LK,Suske G,Beato M,Norén O,Sjöström H (1993) Uteroglobin, an apically secreted protein of the uterine epithelium, is secreted non-polarized form MDCK cells and mainly basolaterally from Caco-2 cells., FEBS Lett, 330, 293-6. [PubMed] [DOI]
Evidence level	Functional assay
Organ	Kidney
Cell	MDCK cells (distal tubule)
Notes	A proteolytic activity assay was utilized

AMPN_PIG (Anpep)

Species	Sus scrofa
Localization	Apical plasma membrane
Reference	Michael Danielsen E,Hansen GH (2016) Small molecule pinocytosis and clathrin-dependent endocytosis at the intestinal brush border: Two separate pathways into the enterocyte., Biochim Biophys Acta, 1858, 233-43. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Small intestine
Cell	Enterocytes
Notes

For ortholog entries:

AMPN_HUMAN

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	10	Cytoplasmic
11	32	Membrane
33	963	Extracellular

Glycosylation

Position	Site resource	PubMed link(s)
82	UniProtKB	22876187
82	dbPTM	22876187
82	PDB	28393915
82	PDB	28393915
82	PDB	22876187
124	PDB	22876187
124	PDB	28393915
124	PDB	28393915
124	dbPTM	22876187
124	UniProtKB	22876187
229	PDB	28393915
229	PDB	28393915
229	PDB	22876187
229	dbPTM	22876187
229	UniProtKB	22876187
237	dbPTM	22876187
237	PDB	28393915
237	PDB	22876187
237	PDB	28393915
237	UniProtKB	22876187
286	PDB	22876187
286	PDB	28393915
314	PDB	28393915
314	PDB	22876187
314	PDB	28393915
314	dbPTM	22876187
314	UniProtKB	22876187
328	dbPTM	22876187
328	PDB	22876187
328	PDB	28393915
328	PDB	28393915
328	UniProtKB	22876187
506	UniProtKB	22876187
506	PDB	22876187
506	PDB	28393915
506	PDB	28393915
506	dbPTM	22876187
622	UniProtKB	22876187
622	dbPTM	22876187
622	PDB	28393915
622	PDB	22876187
622	PDB	28393915
646	PDB	28393915
646	PDB	22876187
646	PDB	28393915
646	dbPTM	22876187
646	UniProtKB	22876187
736	PDB	22876187
736	dbPTM	22876187
736	UniProtKB	22876187

Family and domain data

Database	Domain/Family name
InterPro	Aminopeptidase_N-like_N
InterPro	ERAP1-like_C_dom
InterPro	M1_APN-typ
InterPro	Peptidase_M1
InterPro	Peptidase_M1_dom
Pfam	ERAP1_C
Pfam	Peptidase_M1