PolarProtDb

Gene name

SLC29A1 (Ent1)

Experiment(s)

For this entry:

S29A1_HUMAN (SLC29A1)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Mangravite LM,Xiao G,Giacomini KM (2003) Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells., Am J Physiol Renal Physiol, 284, F902-10. [PubMed] [DOI]
Evidence level	Fluorescent tagging
Organ	Kidney
Cell	MDCK cells (distal tubule)
Notes

S29A1_HUMAN (SLC29A1)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Klein DM,Evans KK,Hardwick RN,Dantzler WH,Wright SH,Cherrington NJ (2013) Basolateral uptake of nucleosides by Sertoli cells is mediated primarily by equilibrative nucleoside transporter 1., J Pharmacol Exp Ther, 346, 121-9. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Testis
Cell	Sertoli cells
Notes

For ortholog entries:

S29A1_RAT

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	12	Cytoplasmic
13	33	Membrane
34	77	Extracellular
78	98	Membrane
99	111	Cytoplasmic
112	132	Membrane
133	138	Extracellular
139	159	Membrane
160	174	Cytoplasmic
175	195	Membrane
196	205	Extracellular
206	226	Membrane
227	290	Cytoplasmic
291	311	Membrane
312	326	Extracellular
327	344	Membrane
345	359	Cytoplasmic
360	380	Membrane
381	395	Extracellular
396	416	Membrane
417	431	Cytoplasmic
432	452	Membrane
453	456	Extracellular

Phosphorylation

Position	Resource
63	25311433
143	18015845
248	50318714
254	486282
266	21326703
269	30991061
271	31884785
273	21326704
281	21326706

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
48	Glyconnect	$WURCS=2.0/2,13,12/[axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-1-1-1-1-2-2-2-2-2-2-2/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?$	29741879
48	Glyconnect	$WURCS=2.0/3,11,10/[axxxxm-1x_1-5][axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-1-2-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?$	29741879
48	dbPTM		19349973
48	N-GlycoSiteAtlas		26571101
48	PolarProtDb		31844046
48	UniPep
48	UniProtKB		19349973
63	PolarProtDb		30350625
63	UniCarbKB		30459171
63	UniCarbKB	$WURCS=2.0/2,2,1/[uxxxxh_2*NCC/3=O][uxxxxh]/1-2/a?\|b?}-{a?\|b?$	30459171
63	Glyconnect		18984734
63	PhosphoSitePlus
63	PolarProtDb		30350625
63	PolarProtDb		29296958
63	dbPTM		50622867
63	O-GlycoProteomDB

Family and domain data

Database	Domain/Family name
InterPro	ENT1
InterPro	ENT1/ENT2
InterPro	Eqnu_transpt
InterPro	MFS_trans_sf
Pfam	Nucleoside_tran

Interactions

With (links to EBI)	#Exp	PubMed links
TMM17_HUMAN	2	26638075

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.