Gene name
Slc12a1 (Nkcc2)
Experiment(s)
For this entry:
S12A1_MOUSE (Slc12a1)
| Species | Mus musculus |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Borschewski A,Himmerkus N,Boldt C,Blankenstein KI,McCormick JA,Lazelle R,Willnow TE,Jankowski V,Plain A,Bleich M,Ellison DH,Bachmann S,Mutig K (2016) Calcineurin and Sorting-Related Receptor with A-Type Repeats Interact to Regulate the Renal Na⁺-K⁺-2Cl⁻ Cotransporter., J Am Soc Nephrol, 27, 107-19. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Kidney |
| Cell | Henle's loop / TAL cells |
| Notes |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 178 | Cytoplasmic |
| 179 | 200 | Membrane |
| 201 | 202 | Extracellular |
| 203 | 230 | Membrane |
| 231 | 251 | Cytoplasmic |
| 252 | 275 | Membrane |
| 276 | 296 | Extracellular |
| 297 | 316 | Membrane |
| 317 | 323 | Cytoplasmic |
| 324 | 341 | Membrane |
| 342 | 375 | Extracellular |
| 376 | 395 | Membrane |
| 396 | 411 | Cytoplasmic |
| 412 | 434 | Membrane |
| 435 | 484 | Extracellular |
| 485 | 506 | Membrane |
| 507 | 542 | Cytoplasmic |
| 543 | 560 | Membrane |
| 561 | 562 | Extracellular |
| 563 | 585 | Membrane |
| 586 | 601 | Cytoplasmic |
| 602 | 619 | Membrane |
| 620 | 621 | Extracellular |
| 622 | 640 | Membrane |
| 641 | 1095 | Cytoplasmic |
Phosphorylation
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | AA-permease/SLC12A_dom |
| InterPro | AA_permease_N |
| InterPro | SLC12_C |
| InterPro | Slc12a1 |
| InterPro | SLC12A1/SLC12A2 |
| InterPro | SLC12A_fam |
| Pfam | AA_permease |
| Pfam | AA_permease_N |
| Pfam | SLC12 |
Interactions
| With (links to EBI) | #Exp | PubMed links |
|---|---|---|
| ALDOB_HUMAN | 2 | 17848580 |
Protein can be found in apical membrane,
Protein can be found in basal membrane,
Protein can be found both membranes,
Localisation is unknown.