Gene name
ALPP (PLAP)
Experiment(s)
For this entry:
PPB1_HUMAN (ALPP)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Benting JH,Rietveld AG,Simons K (1999) N-Glycans mediate the apical sorting of a GPI-anchored, raft-associated protein in Madin-Darby canine kidney cells., J Cell Biol, 146, 313-20. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Kidney |
| Cell | MDCK cells (distal tubule) |
| Notes |
PPB1_HUMAN (ALPP)
| Species | Homo sapiens |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Lipardi C,Nitsch L,Zurzolo C (2000) Detergent-insoluble GPI-anchored proteins are apically sorted in fischer rat thyroid cells, but interference with cholesterol or sphingolipids differentially affects detergent insolubility and apical sorting., Mol Biol Cell, 11, 531-42. [PubMed] [DOI] |
| Evidence level | Membrane preparation |
| Organ | Thyroid gland |
| Cell | FRT cells (follicular cells) |
| Notes | GPI anchor and cholesterol rafts are necessary for apical sorting |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 535 | Extracellular |
Phosphorylation
| Position | Resource |
|---|---|
| 53 | 54208892 |
| 114 | 3179010 |
| 117 | 3179013 |
| 177 | 36132694 |
| 182 | 55369709 |
| 191 | 15425047 |
| 199 | 55369712 |
| 234 | 55369715 |
| 239 | 55369718 |
| 243 | 55369721 |
| 244 | 25282436 |
| 273 | 13309967 |
| 314 | 55369724 |
| 367 | 55369727 |
| 368 | 55369729 |
| 372 | 55369732 |
| 377 | 55369735 |
| 410 | 55369738 |
| 411 | 55369741 |
| 415 | 18006582 |
| 421 | 18006585 |
| 432 | 38487480 |
| 434 | 38487483 |
| 436 | 55369744 |
| 438 | 38487486 |
| 441 | 55369747 |
| 445 | 55369750 |
| 453 | 55369753 |
Glycosylation
| Position | Site resource | Glycan resource | PubMed link(s) |
|---|---|---|---|
| 144 | PDB |
![WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1](data:image/png;base64,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)
|
20693656 |
| 144 | PDB |
|
20693656 |
| 144 | PDB |
![WURCS=2.0/1,1,0/[a2122h-1b_1-5_2*NCC/3=O]/1/](data:image/png;base64,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)
|
15946677 |
| 144 | PDB |
|
15946677 |
| 144 | PDB |
|
15946677 |
| 144 | PDB |
|
16815919 |
| 144 | dbPTM | 15946677 , 16815919 , 20693656 | |
| 144 | N-GlycoSiteAtlas | 18923192 , 25987888 | |
| 144 | UniProtKB | 15946677 , 16815919 , 20693656 | |
| 144 | PDB |
|
20693656 |
| 271 | UniProtKB | 15946677 , 16815919 , 20693656 | |
| 271 | PolarProtDb | 31844046 | |
| 271 | dbPTM | 15946677 , 16815919 , 20693656 | |
| 271 | PDB |
|
16815919 |
| 271 | PDB |
|
15946677 |
| 271 | PDB |
|
15946677 |
| 271 | PDB |
|
15946677 |
| 271 | PDB |
|
20693656 |
| 271 | PDB |
|
20693656 |
| 271 | PDB |
|
20693656 |
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | Alkaline_phosphatase |
| InterPro | Alkaline_phosphatase_AS |
| InterPro | Alkaline_phosphatase_core_sf |
| Pfam | Alk_phosphatase |
Interactions
| With (links to EBI) | #Exp | PubMed links |
|---|---|---|
| NLGN3_HUMAN | 2 | 25464930 |
| CRTP1_HUMAN | 3 | 32296183 |
| GSC2_HUMAN | 3 | 32296183 |
| RGS20_HUMAN | 3 | 32296183 |
| LCE3B_HUMAN | 3 | 32296183 |
| KRA59_HUMAN | 6 | 25416956 , 32296183 |
| CFTR_HUMAN | 8 | 29924966 |
| PO4F2_HUMAN | 3 | 32296183 |
| IBP6_HUMAN | 3 | 32296183 |
| ATL4_HUMAN | 3 | 32296183 |
| CRCT1_HUMAN | 3 | 32296183 |
| LCE5A_HUMAN | 3 | 32296183 |
| LCE2C_HUMAN | 3 | 32296183 |
| LCE1D_HUMAN | 3 | 32296183 |
| HXA1_HUMAN | 7 | 32296183 |
| MCSP_HUMAN | 3 | 32296183 |
| LELP1_HUMAN | 3 | 32296183 |
| Q6FHY5_HUMAN | 3 | 32296183 |
| LCE3E_HUMAN | 3 | 32296183 |
| TGM1_HUMAN | 3 | 32296183 |
| LCE3A_HUMAN | 5 | 32296183 |
| KR412_HUMAN | 3 | 25416956 |
| LCE3C_HUMAN | 3 | 32296183 |
| CXCL5_HUMAN | 3 | 32296183 |
| KR108_HUMAN | 3 | 32296183 |
| LCE3D_HUMAN | 5 | 32296183 |
| FBX34_HUMAN | 3 | 32296183 |
| KRA11_HUMAN | 3 | 32296183 |
| RGS19_HUMAN | 3 | 32296183 |
| LCE1E_HUMAN | 3 | 32296183 |
| OTX1_HUMAN | 3 | 32296183 |
| NT2NC_HUMAN | 3 | 32296183 |
| LCE1F_HUMAN | 3 | 32296183 |
| CREB5_HUMAN | 3 | 32296183 |
| NR4A3_HUMAN | 3 | 32296183 |
| GEMI4_HUMAN | 3 | 32296183 |
Protein can be found in apical membrane,
Protein can be found in basal membrane,
Protein can be found both membranes,
Localisation is unknown.