PolarProtDb

Gene name

KCNJ4 (IRK3)

Experiment(s)

For this entry:

KCNJ4_MOUSE (KCNJ4)

Species	Mus musculus
Localization	Basolateral plasma membrane
Reference	Le Maout S,Welling PA,Brejon M,Olsen O,Merot J (2001) Basolateral membrane expression of a K+ channel, Kir 2.3, is directed by a cytoplasmic COOH-terminal domain., Proc Natl Acad Sci U S A, 98, 10475-80. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Kidney
Cell	MDCK cells (distal tubule)
Notes	A VSV epitope was used for labelling. Localization was dependent on a PDZ motif

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	53	Cytoplasmic
54	80	Membrane
81	120	Extracellular
121	137	Re-entrant loop
138	145	Extracellular
146	172	Membrane
173	445	Cytoplasmic

Eukaryotic Linear Motif (ELM)

Start	End	Sequence	Motif
12	15	VPRR	TRG_ER_diArg_1

Motifs gathered by PolarProtDb Team

Start	End	Localisation	Type	PubMed
440	445	Basolateral plasma membrane	PDZ-ligand	Le Maout S,Welling PA,Brejon M,Olsen O,Merot J (2001) Basolateral membrane expression of a K+ channel, Kir 2.3, is directed by a cytoplasmic COOH-terminal domain., Proc Natl Acad Sci U S A, 98, 10475-80. [PubMed][DOI]

Phosphorylation

Position	Resource
420	15501683

Family and domain data

Database	Domain/Family name
InterPro	Ig_E-set
InterPro	IRK_C
InterPro	K_chnl_inward-rec_Kir
InterPro	K_chnl_inward-rec_Kir2.3
InterPro	K_chnl_inward-rec_Kir_cyto
InterPro	Kir_TM
Pfam	IRK
Pfam	IRK_C

Interactions

With (links to EBI)	#Exp	PubMed links
DLG4_MOUSE	3	28671696 , 19455133

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.