PolarProtDb

Gene name

KCNMA1 (KCNMA,SLO)

Experiment(s)

For this entry:

KCMA1_HUMAN (KCNMA1)

Species	Homo sapiens
Localization	Apical plasma membrane
Reference	Bravo-Zehnder M,Orio P,Norambuena A,Wallner M,Meera P,Toro L,Latorre R,González A (2000) Apical sorting of a voltage- and Ca2+-activated K+ channel alpha -subunit in Madin-Darby canine kidney cells is independent of N-glycosylation., Proc Natl Acad Sci U S A, 97, 13114-9. [PubMed] [DOI]
Evidence level	Membrane preparation
Organ	Kidney
Cell	MDCK cells (distal tubule)
Notes	Localization is independent of glycosylation

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	88	Cytoplasmic
89	107	Membrane
108	176	Extracellular
177	197	Membrane
198	213	Cytoplasmic
214	234	Membrane
235	248	Extracellular
249	269	Membrane
270	299	Cytoplasmic
300	320	Membrane
321	336	Extracellular
337	356	Membrane
357	366	Cytoplasmic
367	385	Membrane
386	1236	Extracellular

Phosphorylation

Position	Resource
12	3072551
22	3071423
41	11923404
42	23117608
43	23117610
44	23117613
45	23117616
47	23117619
50	23117622
51	23117625
52	23117628
53	23117631
55	23117634
562	23117637
569	2526007
763	456599
765	456596
778	17548131
782	17548137
978	456598
992	469989
1086	457450
1146	23117596
1149	23117599
1152	23117640
1188	56657388
1196	23117602
1200	9805700
1205	23117605
1208	27721206
1230	23117643

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
780	Glyconnect	$WURCS=2.0/2,12,11/[axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-1-1-1-1-2-2-2-2-2-2/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?$	29741879
780	N-GlycoSiteAtlas		24741114

Family and domain data

Database	Domain/Family name
InterPro	Ca-act_K_channel_Slo-1
InterPro	Ion_trans_dom
InterPro	K_chnl_BK_asu
InterPro	NAD(P)-bd_dom_sf
InterPro	Volt_channel_dom_sf
Pfam	BK_channel_a
Pfam	Ion_trans