PolarProtDb

Gene name

Itga3 (SGCA,ITGA3B,MSK18)

Experiment(s)

For this entry:

ITA3_HUMAN (Itga3)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Chin AR,Yan W,Cao M,Liu X,Wang SE (2018) Polarized Secretion of Extracellular Vesicles by Mammary Epithelia., J Mammary Gland Biol Neoplasia, 23, 165-176. [PubMed] [DOI]
Evidence level	Large-scale proteomics
Organ	Mammary gland
Cell	MCF10A cells (acinar cells)
Notes	Detected in the polarized exosome fraction

For ortholog entries:

F1Q439_CANLF

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	33	Signal peptide
34	1000	Extracellular
1001	1019	Membrane
1020	1051	Cytoplasmic

Phosphorylation

Position	Resource
150	55371408
237	55371410
238	55371412
574	975378
575	23116527
793	55371414
936	25296355
937	25296359
1028	15404077
1042	447602
1044	473572
1048	5473788
1051	457834

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
86	N-GlycoSiteAtlas		24495048
107	N-GlycoSiteAtlas		22457533 , 25247386 , 24495048 , 26256267 , 25902405
127	O-GlycoProteomDB
247	dbPTM		16399764
265	N-GlycoSiteAtlas		17506567 , 17074749 , 19530676 , 19530676 , 19530676 , 19530676 , 19196183 , 18923192 , 22113853 , 21621025 , 22457533 , 22106898 , 24164404 , 24324730 , 25285165 , 24741114 , 24721674 , 24190977 , 25109867 , 25244057 , 25247386 , 24495048 , 26571101 , 26256267 , 25902405
265	PolarProtDb		31844046
265	UniPep
500	N-GlycoSiteAtlas		26571101
511	N-GlycoSiteAtlas		17506567 , 22106898
573	N-GlycoSiteAtlas		26571101
573	UniPep
605	N-GlycoSiteAtlas		26571101
605	UniPep
656	Glyconnect	$WURCS=2.0/2,5,4/[axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-2-2-2/a?\|b?\|c?\|d?\|e?}-{a?\|b?\|c?\|d?\|e?_a?\|b?\|c?\|d?\|e?}-{a?\|b?\|c?\|d?\|e?_a?\|b?\|c?\|d?\|e?}-{a?\|b?\|c?\|d?\|e?_a?\|b?\|c?\|d?\|e?}-{a?\|b?\|c?\|d?\|e?$	23345538
656	PolarProtDb		31844046
656	N-GlycoSiteAtlas		24495048
656	UniCarbKB	$WURCS=2.0/2,5,4/[uxxxxh_2*NCC/3=O][uxxxxh]/1-1-2-2-2/a?\|b?\|c?\|d?\|e?}-{a?\|b?\|c?\|d?\|e?_a?\|b?\|c?\|d?\|e?}-{a?\|b?\|c?\|d?\|e?_a?\|b?\|c?\|d?\|e?}-{a?\|b?\|c?\|d?\|e?_a?\|b?\|c?\|d?\|e?}-{a?\|b?\|c?\|d?\|e?$	23345538
656	Glyconnect	$WURCS=2.0/2,7,6/[axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-2-2-2-2-2/a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?_a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?_a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?_a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?_a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?_a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?$	29741879
697	N-GlycoSiteAtlas		24495048
841	N-GlycoSiteAtlas		26571101
857	N-GlycoSiteAtlas		26571101
889	O-GlycoProteomDB
926	UniCarbKB	$WURCS=2.0/3,8,7/[uxxxxm][uxxxxh_2*NCC/3=O][uxxxxh]/1-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?$	23345538
926	Glyconnect	$WURCS=2.0/3,8,7/[axxxxm-1x_1-5][axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?$	23345538
926	N-GlycoSiteAtlas		23090970
935	N-GlycoSiteAtlas		24495048
969	N-GlycoSiteAtlas		17506567 , 19530676 , 22457533 , 22148984 , 22106898 , 24164404 , 24324730 , 25285165 , 24741114 , 24190977 , 25244057 , 25247386 , 24495048 , 26256267

Family and domain data

Database	Domain/Family name
InterPro	FG-GAP
InterPro	Int_alpha_beta-p
InterPro	Integrin_alpha
InterPro	Integrin_alpha-2
InterPro	Integrin_alpha_C_CS
InterPro	Integrin_alpha_N
InterPro	Integrin_dom_sf
Pfam	FG-GAP
Pfam	Integrin_alpha2

Interactions

With (links to EBI)	#Exp	PubMed links
CD151_HUMAN	6	10811835 , 14557253
CFTR_HUMAN	7	29924966
CREL2_MOUSE	2	23956175

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.