PolarProtDb

Gene name

IL6R

Experiment(s)

For this entry:

IL6RA_HUMAN (IL6R)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Martens AS,Bode JG,Heinrich PC,Graeve L (2000) The cytoplasmic domain of the interleukin-6 receptor gp80 mediates its basolateral sorting in polarized madin-darby canine kidney cells., J Cell Sci, 113 ( Pt 20), 3593-602. [PubMed]
Evidence level	Antibody staining + sub-protein manipulation
Organ	Kidney
Cell	MDCK cells (distal tubule)
Notes	The basolateral determinant resided somwehere in the cytoplasmic segment

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	19	Signal peptide
20	365	Extracellular
366	386	Membrane
387	468	Cytoplasmic

Phosphorylation

Position	Resource
91	34367009
139	8090365
141	8090369
144	12069504
243	23115610
455	23115612
457	463588
464	463589

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
55	UniProtKB		10066782 , 28060820
55	N-GlycoSiteAtlas		24495048
55	dbPTM		10066782
55	PDB		12461182
93	UniProtKB		10066782 , 28060820
93	UniPep
93	PolarProtDb		31844046
93	N-GlycoSiteAtlas		24495048
93	dbPTM		10066782
93	PDB		12461182
221	PDB		28134246
221	UniProtKB		10066782 , 28060820
221	N-GlycoSiteAtlas		26571101
221	dbPTM		10066782
221	PDB		12461182
245	UniProtKB		28060820
245	N-GlycoSiteAtlas		24495048
245	PDB		28134246
245	PDB		12461182
329	O-GlycoProteomDB
329	UniCarbKB	$WURCS=2.0/2,2,1/[uxxxxh_2*NCC/3=O][uxxxxh]/1-2/a?\|b?}-{a?\|b?$	30459171
335	UniCarbKB	$WURCS=2.0/2,4,3/[uxxxxh_2*NCC/3=O][uxxxxh]/1-1-2-2/a?\|b?\|c?\|d?}-{a?\|b?\|c?\|d?_a?\|b?\|c?\|d?}-{a?\|b?\|c?\|d?_a?\|b?\|c?\|d?}-{a?\|b?\|c?\|d?$	30459171
350	UniPep
350	UniProtKB		28060820
352	UniProtKB		28060820

Family and domain data

Database	Domain/Family name
InterPro	FN3_dom
InterPro	FN3_sf
InterPro	Hematopoietin_rcpt_L_F3_CS
InterPro	Ig-like_dom
InterPro	Ig-like_dom_sf
InterPro	Ig-like_fold
InterPro	Ig_sub
InterPro	Ig_sub2
InterPro	Immunoglobulin
InterPro	TypeI_recpt_CBD
Pfam	ig
Pfam	IL6Ra-bind

Interactions

With (links to EBI)	#Exp	PubMed links
SGTB_HUMAN	3	32296183
IL6_HUMAN	3	18775332 , 12829785 , 28265003
SORL_HUMAN	7	28265003

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.