PolarProtDb

Gene name

IGFBP2 (BP2,IBP2,Igfbp-2)

Experiment(s)

For this entry:

IBP2_HUMAN (IGFBP2)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Remacle-Bonnet M,Garrouste F,el Atiq F,Marvaldi J,Pommier G (1995) Cell polarity of the insulin-like growth factor system in human intestinal epithelial cells. Unique apical sorting of insulin-like growth factor binding protein-6 in differentiated human colon cancer cells., J Clin Invest, 96, 192-200. [PubMed] [DOI]
Evidence level	Selective medium analysis
Organ	Large intestine
Cell	HT29-D4 cells (enterocytes)
Notes	Endogenous protein was detected

IBP2_HUMAN (IGFBP2)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Touzelet O,Broadbent L,Armstrong SD,Aljabr W,Cloutman-Green E,Power UF,Hiscox JA (2020) The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins., Mol Cell Proteomics, 19, 793-807. [PubMed] [DOI]
Evidence level	Large-scale proteomics
Organ	Lung
Cell	Airway epithel cells
Notes	Only mock-transfected cells were considered

IBP2_HUMAN (IGFBP2)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Touzelet O,Broadbent L,Armstrong SD,Aljabr W,Cloutman-Green E,Power UF,Hiscox JA (2020) The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins., Mol Cell Proteomics, 19, 793-807. [PubMed] [DOI]
Evidence level	Large-scale proteomics
Organ	Lung
Cell	Airway epithel cells
Notes

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	325	Extracellular

Phosphorylation

Position	Resource
142	1309500
196	23115173
299	33526396

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
165	O-GlycoProteomDB
166	UniCarbKB	$WURCS=2.0/2,2,1/[uxxxxh_2*NCC/3=O][uxxxxh]/1-2/a?\|b?}-{a?\|b?$	30459171
196	O-GlycoProteomDB
299	O-GlycoProteomDB
321	PolarProtDb		29296958
321	O-GlycoProteomDB

Family and domain data

Database	Domain/Family name
InterPro	Growth_fac_rcpt_cys_sf
InterPro	IGFBP-2
InterPro	IGFBP-like
InterPro	IGFBP_1-6_chordata
InterPro	Insulin_GF-bd_Cys-rich_CS
InterPro	Thyroglobulin_1
InterPro	Thyroglobulin_1_sf
Pfam	IGFBP
Pfam	Thyroglobulin_1

Interactions

With (links to EBI)	#Exp	PubMed links
IN80B_HUMAN	4	19095771

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.