PolarProtDb

Gene name

Furin (Fur,PACE,Pcsk3)

Experiment(s)

For this entry:

FURIN_HUMAN (Furin)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Simmen T,Nobile M,Bonifacino JS,Hunziker W (1999) Basolateral sorting of furin in MDCK cells requires a phenylalanine-isoleucine motif together with an acidic amino acid cluster., Mol Cell Biol, 19, 3136-44. [PubMed] [DOI]
Evidence level	Antibody staining + sub-protein manipulation
Organ	Kidney
Cell	MDCK cells (distal tubule)
Notes	A Tyrosine-based (YKGL) and a dileucine-type motif (ERTAFI) were responsible for basolateral targeting

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	24	Signal peptide
25	715	Extracellular
716	736	Membrane
737	794	Cytoplasmic

Motifs gathered by PolarProtDb Team

Start	End	Localisation	Type	PubMed
758	763	Basolateral plasma membrane	Tyrosine	Simmen T,Nobile M,Bonifacino JS,Hunziker W (1999) Basolateral sorting of furin in MDCK cells requires a phenylalanine-isoleucine motif together with an acidic amino acid cluster., Mol Cell Biol, 19, 3136-44. [PubMed][DOI]
782	788	Basolateral plasma membrane	Dileucine-like	Simmen T,Nobile M,Bonifacino JS,Hunziker W (1999) Basolateral sorting of furin in MDCK cells requires a phenylalanine-isoleucine motif together with an acidic amino acid cluster., Mol Cell Biol, 19, 3136-44. [PubMed][DOI]

Phosphorylation

Position	Resource
123	23108381
167	3919951
625	34500942
773	469742
775	469743

Glycosylation

Position	Site resource	PubMed link(s)
387	PDB	12794637
387	PDB	27670069
440	PDB	27670069
440	N-GlycoSiteAtlas
553	N-GlycoSiteAtlas
685	O-GlycoProteomDB

Family and domain data

Database	Domain/Family name
InterPro	Furin_repeat
InterPro	Galactose-bd-like_sf
InterPro	Growth_fac_rcpt_cys_sf
InterPro	Kexin/furin
InterPro	P_dom
InterPro	Peptidase_S8/S53_dom
InterPro	Peptidase_S8/S53_dom_sf
InterPro	Peptidase_S8_Asp-AS
InterPro	Peptidase_S8_His-AS
InterPro	Peptidase_S8_Ser-AS
InterPro	Peptidase_S8_subtilisin-rel
InterPro	S8_pro-domain
InterPro	S8_pro-domain_sf
Pfam	P_proprotein
Pfam	Peptidase_S8
Pfam	S8_pro-domain

Interactions

With (links to EBI)	#Exp	PubMed links
MMP28_HUMAN	3	21152186
SPIKE_MERS1	2	unassigned2497
MMP14_HUMAN	2	21152186

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.