PolarProtDb

Gene name

Enpp1 (M6S1,Npps,Pc1,Pdnp1)

Experiment(s)

For this entry:

ENPP1_MOUSE (Enpp1)

Species	Mus musculus
Localization	Basolateral plasma membrane
Reference	Bello V,Goding JW,Greengrass V,Sali A,Dubljevic V,Lenoir C,Trugnan G,Maurice M (2001) Characterization of a di-leucine-based signal in the cytoplasmic tail of the nucleotide-pyrophosphatase NPP1 that mediates basolateral targeting but not endocytosis., Mol Biol Cell, 12, 3004-15. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Kidney
Cell	MDCK cells (distal tubule)
Notes	A non-canonical dileucine motif was responsible for basolateral sorting

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	58	Cytoplasmic
59	79	Membrane
80	906	Extracellular

Phosphorylation

Position	Resource
25	4701736
238	451402

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
267	PDB		23027977
267	PDB		23027977
267	PDB		23027977
267	PDB		23027977
267	PDB		30356045
267	PDB		30356045
267	dbPTM		19656770 , 23027977
267	UniProtKB		19656770 , 23027977
267	PDB		23041369
323	UniProtKB		19656770 , 23027977 , 23041369
323	PolarProtDb		31844046
323	dbPTM		19656770 , 23027977 , 23041369
323	PDB		30356045
323	PDB		30356045
323	PDB		23027977
323	PDB		23027977
323	PDB		23027977
323	PDB		23041369
323	PDB		23027977
459	Glyconnect	$WURCS=2.0/2,7,6/[axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-2-2-2-2-2/a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?_a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?_a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?_a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?_a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?_a?\|b?\|c?\|d?\|e?\|f?\|g?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?$	30899004
459	PDB		23041369
459	PDB		30356045
459	PDB		30356045
459	dbPTM		23041369
459	UniProtKB		23041369
567	PDB		23041369
567	UniProtKB		23027977 , 23041369
567	dbPTM		23027977 , 23041369
567	PDB		30356045
567	PDB		30356045
567	PDB		23027977
567	PDB		23027977
567	PDB		23027977
567	PDB		23027977
624	dbPTM		19656770 , 23041369
624	UniProtKB		19656770 , 23041369

Family and domain data

Database	Domain/Family name
InterPro	Alkaline_phosphatase_core_sf
InterPro	DNA/RNA_non-sp_Endonuclease
InterPro	ENPP1
InterPro	Extracellular_endonuc_su_A
InterPro	Phosphodiest/P_Trfase
InterPro	Somatomedin_B-like_dom_sf
InterPro	Somatomedin_B_chordata
InterPro	Somatomedin_B_dom
Pfam	Endonuclease_NS
Pfam	Phosphodiest
Pfam	Somatomedin_B