Gene name
SLC1A3 (GLAST,EAAT1,Gmt1,ECO:0000303|PubMed:8647279},GLAST1)
Experiment(s)
For this entry:
EAA1_HUMAN (SLC1A3)
| Species | Homo sapiens |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Cheng C,Glover G,Banker G,Amara SG (2002) A novel sorting motif in the glutamate transporter excitatory amino acid transporter 3 directs its targeting in Madin-Darby canine kidney cells and hippocampal neurons., J Neurosci, 22, 10643-52. [PubMed] |
| Evidence level | Fluorescent tagging |
| Organ | Kidney |
| Cell | MDCK cells (distal tubule) |
| Notes | The source of plasmids is not mentioned but likely human |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 47 | Cytoplasmic |
| 48 | 68 | Membrane |
| 69 | 90 | Extracellular |
| 91 | 111 | Membrane |
| 112 | 122 | Cytoplasmic |
| 123 | 144 | Membrane |
| 145 | 239 | Extracellular |
| 240 | 260 | Membrane |
| 261 | 280 | Cytoplasmic |
| 281 | 301 | Membrane |
| 302 | 317 | Extracellular |
| 318 | 338 | Membrane |
| 339 | 393 | Cytoplasmic |
| 394 | 414 | Membrane |
| 415 | 439 | Extracellular |
| 440 | 467 | Membrane |
| 468 | 542 | Cytoplasmic |
Eukaryotic Linear Motif (ELM)
| Start | End | Sequence | Motif |
|---|---|---|---|
| 538 | 542 | SETKM | TRG_PTS1 |
Phosphorylation
Glycosylation
| Position | Site resource | Glycan resource | PubMed link(s) |
|---|---|---|---|
| 206 | N-GlycoSiteAtlas | 26571101 | |
| 206 | PolarProtDb | 31844046 | |
| 216 | N-GlycoSiteAtlas | 26571101 | |
| 216 | PolarProtDb | 31844046 |
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | Na-dicarboxylate_symporter |
| InterPro | Na-dicarboxylate_symporter_CS |
| InterPro | Na:dicarbo_symporter_sf |
| Pfam | SDF |
Interactions
| With (links to EBI) | #Exp | PubMed links |
|---|---|---|
| T120B_HUMAN | 3 | 32296183 |
| TM128_HUMAN | 3 | 32296183 |
| MGST3_HUMAN | 3 | 32296183 |
| BT2A2_HUMAN | 3 | 32296183 |
| TMM17_HUMAN | 2 | 26638075 |
Protein can be found in apical membrane,
Protein can be found in basal membrane,
Protein can be found both membranes,
Localisation is unknown.