PolarProtDb

Gene name

CD55 (CR,DAF)

Experiment(s)

For this entry:

DAF_HUMAN (CD55)

Species	Homo sapiens
Localization	Apical plasma membrane
Reference	Lawrence DW,Bruyninckx WJ,Louis NA,Lublin DM,Stahl GL,Parkos CA,Colgan SP (2003) Antiadhesive role of apical decay-accelerating factor (CD55) in human neutrophil transmigration across mucosal epithelia., J Exp Med, 198, 999-1010. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Large intestine
Cell	T84 cells (enterocytes)
Notes

DAF_HUMAN (CD55)

Species	Homo sapiens
Localization	Non polar membrane
Reference	Ma KN,Cashman SM,Sweigard JH,Kumar-Singh R (2010) Decay accelerating factor (CD55)-mediated attenuation of complement: therapeutic implications for age-related macular degeneration., Invest Ophthalmol Vis Sci, 51, 6776-83. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Retina
Cell	Pigment epithel cells
Notes	Chimeric proteins were used. N-glycans were necessary for apical delivery

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	381	Extracellular

Phosphorylation

Position	Resource
140	18007759
162	6151066
245	23091991
251	3700161
266	15408596
310	36128603
334	23091988

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
95	UniProtKB		19159218
95	UniPep
95	N-GlycoSiteAtlas		24495048
95	dbPTM		19159218
95	Glyconnect	$WURCS=2.0/4,11,10/[Aad21122h-2x_2-6_5NCC/3=O][axxxxm-1x_1-5][axxxxh-1x_1-5_2NCC/3=O][axxxxh-1x_1-5]/1-2-3-3-3-3-4-4-4-4-4/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?$	29741879
95	Glyconnect	$WURCS=2.0/4,12,11/[Aad21122h-2x_2-6_5NCC/3=O][axxxxm-1x_1-5][axxxxh-1x_1-5_2NCC/3=O][axxxxh-1x_1-5]/1-2-2-3-3-3-3-4-4-4-4-4/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?$	29741879
95	Glyconnect	$WURCS=2.0/3,11,10/[Aad21122h-2x_2-6_5NCC/3=O][axxxxh-1x_1-5_2NCC/3=O][axxxxh-1x_1-5]/1-1-2-2-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?$	29741879
95	Glyconnect	$WURCS=2.0/3,12,11/[axxxxm-1x_1-5][axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-1-2-2-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?$	29741879
95	Glyconnect	$WURCS=2.0/3,10,9/[axxxxm-1x_1-5][axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-2-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?$	29741879
95	Glyconnect	$WURCS=2.0/3,10,9/[Aad21122h-2x_2-6_5NCC/3=O][axxxxh-1x_1-5_2NCC/3=O][axxxxh-1x_1-5]/1-2-2-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?$	29741879
289	O-GlycoProteomDB
290	O-GlycoProteomDB
295	O-GlycoProteomDB
300	O-GlycoProteomDB
301	O-GlycoProteomDB
306	O-GlycoProteomDB
307	O-GlycoProteomDB
312	O-GlycoProteomDB
313	O-GlycoProteomDB
320	O-GlycoProteomDB
321	O-GlycoProteomDB
322	O-GlycoProteomDB
328	O-GlycoProteomDB
330	O-GlycoProteomDB
331	O-GlycoProteomDB
334	O-GlycoProteomDB
336	O-GlycoProteomDB
337	O-GlycoProteomDB
343	O-GlycoProteomDB
343	dbPTM		55827263
343	PhosphoSitePlus
344	O-GlycoProteomDB
344	dbPTM		55827267
344	PhosphoSitePlus
349	O-GlycoProteomDB
351	O-GlycoProteomDB
352	O-GlycoProteomDB
353	O-GlycoProteomDB
355	O-GlycoProteomDB
356	O-GlycoProteomDB

Family and domain data

Database	Domain/Family name
InterPro	Sushi/SCR/CCP_sf
InterPro	Sushi_SCR_CCP_dom
Pfam	Sushi

Interactions

With (links to EBI)	#Exp	PubMed links
Q914E0_9ENTO	3	12119400
CFTR_HUMAN	5	29924966
ESR1_HUMAN	2	31527615

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.