PolarProtDb

Gene name

CXADR (Car)

Experiment(s)

For this entry:

CXAR_HUMAN (CXADR)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Carvajal-Gonzalez JM,Gravotta D,Mattera R,Diaz F,Perez Bay A,Roman AC,Schreiner RP,Thuenauer R,Bonifacino JS,Rodriguez-Boulan E (2012) Basolateral sorting of the coxsackie and adenovirus receptor through interaction of a canonical YXXPhi motif with the clathrin adaptors AP-1A and AP-1B., Proc Natl Acad Sci U S A, 109, 3820-5. [PubMed] [DOI]
Evidence level	Fluorescent tagging + sub-protein manipulation
Organ	Kidney
Cell	MDCK cells (distal tubule)
Notes	The source of plasmid was not mentioned explicitly. A Tyr-based motif was confirmed to direct sorting.

CXAR_HUMAN (CXADR)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Walters RW,Grunst T,Bergelson JM,Finberg RW,Welsh MJ,Zabner J (1999) Basolateral localization of fiber receptors limits adenovirus infection from the apical surface of airway epithelia., J Biol Chem, 274, 10219-26. [PubMed] [DOI]
Evidence level	Functional assay
Organ	Lung
Cell	Airway epithel cells
Notes	Viral infectivity assays were used in addition to antibody labellings of endogenous protein

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	19	Signal peptide
20	242	Extracellular
243	259	Membrane
260	365	Cytoplasmic

Motifs gathered by PolarProtDb Team

Start	End	Localisation	Type	PubMed
317	322	Basolateral plasma membrane	Tyrosine	Carvajal-Gonzalez JM,Gravotta D,Mattera R,Diaz F,Perez Bay A,Roman AC,Schreiner RP,Thuenauer R,Bonifacino JS,Rodriguez-Boulan E (2012) Basolateral sorting of the coxsackie and adenovirus receptor through interaction of a canonical YXXPhi motif with the clathrin adaptors AP-1A and AP-1B., Proc Natl Acad Sci U S A, 109, 3820-5. [PubMed][DOI]

Phosphorylation

Position	Resource
100	23097031
233	18909763
288	12219664
290	801662702
293	3193535
294	462043
297	4713440
300	4713445
301	4713450
304	4713455
306	3193538
308	31888128
313	462044
314	12477718
318	455154
323	467714
329	31880363
332	452735
334	4713460
346	50776650
359	4713465
363	4713470

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
106	Glyconnect	$WURCS=2.0/3,11,10/[Aad21122h-2x_2-6_5NCC/3=O][axxxxh-1x_1-5_2NCC/3=O][axxxxh-1x_1-5]/1-1-2-2-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?$	29741879
106	N-GlycoSiteAtlas		24495048
106	PolarProtDb		31844046
201	N-GlycoSiteAtlas		24495048
201	PolarProtDb		31844046

Family and domain data

Database	Domain/Family name
InterPro	Ig-like_dom
InterPro	Ig-like_dom_sf
InterPro	Ig-like_fold
InterPro	Ig_sub
InterPro	Ig_sub2
InterPro	Ig_V-set
InterPro	Immunoglobulin
Pfam	ig
Pfam	V-set

Interactions

With (links to EBI)	#Exp	PubMed links
MAL_HUMAN	3	32296183
POLG_CXB3W	4	11573093
ANR46_HUMAN	3	32296183
FAM3C_HUMAN	3	32296183
TMM17_HUMAN	2	26638075
TM216_HUMAN	2	26638075

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.