PolarProtDb

Gene name

CA12 (Car12)

Experiment(s)

For this entry:

CAH12_HUMAN (CA12)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Hermo L,Chong DL,Moffatt P,Sly WS,Waheed A,Smith CE (2005) Region- and cell-specific differences in the distribution of carbonic anhydrases II, III, XII, and XIV in the adult rat epididymis., J Histochem Cytochem, 53, 699-713. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Epididymis
Cell	Principal cells
Notes

CAH12_HUMAN (CA12)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Lee M,Vecchio-Pagán B,Sharma N,Waheed A,Li X,Raraigh KS,Robbins S,Han ST,Franca AL,Pellicore MJ,Evans TA,Arcara KM,Nguyen H,Luan S,Belchis D,Hertecant J,Zabner J,Sly WS,Cutting GR (2016) Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease., Hum Mol Genet, 25, 1923-1933. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Kidney
Cell	MDCK cells (distal tubule)
Notes

CAH12_HUMAN (CA12)

Species	Homo sapiens
Localization	Apical plasma membrane
Reference	Lee M,Vecchio-Pagán B,Sharma N,Waheed A,Li X,Raraigh KS,Robbins S,Han ST,Franca AL,Pellicore MJ,Evans TA,Arcara KM,Nguyen H,Luan S,Belchis D,Hertecant J,Zabner J,Sly WS,Cutting GR (2016) Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease., Hum Mol Genet, 25, 1923-1933. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Lung
Cell	Airway epithel cells
Notes

CAH12_HUMAN (CA12)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Lee M,Vecchio-Pagán B,Sharma N,Waheed A,Li X,Raraigh KS,Robbins S,Han ST,Franca AL,Pellicore MJ,Evans TA,Arcara KM,Nguyen H,Luan S,Belchis D,Hertecant J,Zabner J,Sly WS,Cutting GR (2016) Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease., Hum Mol Genet, 25, 1923-1933. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Sweat glands
Cell	Ductal cells
Notes

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	24	Signal peptide
25	309	Extracellular
310	327	Membrane
328	354	Cytoplasmic

Phosphorylation

Position	Resource
342	1011315

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
80	Glyconnect	$WURCS=2.0/3,14,13/[Aad21122h-2x_2-6_5NCC/3=O][axxxxh-1x_1-5_2NCC/3=O][axxxxh-1x_1-5]/1-1-1-2-2-2-2-2-3-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?\|n?$	29741879
80	N-GlycoSiteAtlas
80	PolarProtDb		31844046
80	UniPep

Family and domain data

Database	Domain/Family name
InterPro	CA_dom
InterPro	CA_dom_sf
InterPro	Carbonic_anhydrase_a-class
InterPro	Carbonic_anhydrase_a-class_CS
InterPro	Carbonic_anhydrase_CA12
Pfam	Carb_anhydrase