PolarProtDb

Gene name

ATP1B2

Experiment(s)

For this entry:

AT1B2_HUMAN (ATP1B2)

Species	Homo sapiens
Localization	Apical plasma membrane
Reference	Vagin O,Turdikulova S,Sachs G (2005) Recombinant addition of N-glycosylation sites to the basolateral Na,K-ATPase beta1 subunit results in its clustering in caveolae and apical sorting in HGT-1 cells., J Biol Chem, 280, 43159-67. [PubMed] [DOI]
Evidence level	Membrane preparation
Organ	Stomach
Cell	HGT-1 cells (parietal cells)
Notes	Endogenous protein detected by selective biotinylation

AT1B2_HUMAN (ATP1B2)

Species	Homo sapiens
Localization	Apical plasma membrane
Reference	Lobato-Álvarez JA,Roldán ML,López-Murillo TD,González-Ramírez R,Bonilla-Delgado J,Shoshani L (2016) The Apical Localization of Na<sup>+</sup>, K<sup>+</sup>-ATPase in Cultured Human Retinal Pigment Epithelial Cells Depends on Expression of the β<sub>2</sub> Subunit., Front Physiol, 7, 450. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Retina
Cell	Pigment epithel cells
Notes

AT1B2_HUMAN (ATP1B2)

Species	Homo sapiens
Localization	Apical plasma membrane
Reference	Lobato-Álvarez JA,Roldán ML,López-Murillo TD,González-Ramírez R,Bonilla-Delgado J,Shoshani L (2016) The Apical Localization of Na<sup>+</sup>, K<sup>+</sup>-ATPase in Cultured Human Retinal Pigment Epithelial Cells Depends on Expression of the β<sub>2</sub> Subunit., Front Physiol, 7, 450. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Retina
Cell	ARPE-19 cells (pigment epithel cells)
Notes	Only expressed at the mature stage. Directs K+/Na+ ATPase polarization to apical side.

For ortholog entries:

AT1B2_RAT

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	37	Cytoplasmic
38	63	Membrane
64	290	Extracellular

Phosphorylation

Position	Resource
134	23084878

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
118	Glyconnect	$WURCS=2.0/3,11,10/[axxxxm-1x_1-5][axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-2-2-2-3-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?$	29741879
118	N-GlycoSiteAtlas		21645671 , 22148984 , 24741114 , 24721674 , 24467213 , 24495048 , 26571101
118	PolarProtDb		31844046
153	N-GlycoSiteAtlas
159	N-GlycoSiteAtlas		24495048
193	PolarProtDb		31844046
193	UniPep
197	PolarProtDb		31844046
197	UniPep
238	dbPTM		19159218
238	N-GlycoSiteAtlas		19159218 , 21645671 , 22148984 , 24721674 , 24495048 , 26571101
238	PolarProtDb		31844046
238	UniProtKB		19159218
250	N-GlycoSiteAtlas		26571101

Family and domain data

Database	Domain/Family name
InterPro	Na/K_ATPase_sub_beta
InterPro	Na/K_ATPase_sub_beta_sf
Pfam	Na_K-ATPase