Gene name
Areg (AREGB,Sdgf)
Experiment(s)
For this entry:
AREG_HUMAN (Areg)
| Species | Homo sapiens |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Gephart JD,Singh B,Higginbotham JN,Franklin JL,Gonzalez A,Fölsch H,Coffey RJ (2011) Identification of a novel mono-leucine basolateral sorting motif within the cytoplasmic domain of amphiregulin., Traffic, 12, 1793-804. [PubMed] [DOI] |
| Evidence level | Fluorescent tagging + sub-protein manipulation |
| Organ | Kidney |
| Cell | MDCK cells (distal tubule) |
| Notes | A monoleucine motif (EERKKLR) was responsible for basolateral targeting |
AREG_HUMAN (Areg)
| Species | Homo sapiens |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Damstrup L,Kuwada SK,Dempsey PJ,Brown CL,Hawkey CJ,Poulsen HS,Wiley HS,Coffey RJ Jr (1999) Amphiregulin acts as an autocrine growth factor in two human polarizing colon cancer lines that exhibit domain selective EGF receptor mitogenesis., Br J Cancer, 80, 1012-9. [PubMed] [DOI] |
| Evidence level | Antibody staining + selective medium analysis |
| Organ | Large intestine |
| Cell | CACO-2 cells (enterocytes) |
| Notes |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 19 | Signal peptide |
| 20 | 198 | Extracellular |
| 199 | 221 | Membrane |
| 222 | 252 | Cytoplasmic |
Motifs gathered by PolarProtDb Team
| Start | End | Localisation | Type | PubMed |
|---|---|---|---|---|
| 236 | 242 | Basolateral plasma membrane | Monoleucine |
Gephart JD,Singh B,Higginbotham JN,Franklin JL,Gonzalez A,Fölsch H,Coffey RJ (2011) Identification of a novel mono-leucine basolateral sorting motif within the cytoplasmic domain of amphiregulin., Traffic, 12, 1793-804. [PubMed][DOI] |
Phosphorylation
Glycosylation
| Position | Site resource | Glycan resource | PubMed link(s) |
|---|---|---|---|
| 30 | PolarProtDb | 31844046 | |
| 32 | O-GlycoProteomDB | ||
| 41 | O-GlycoProteomDB | ||
| 41 | PhosphoSitePlus |
![WURCS=2.0/1,1,0/[a2112h-1x_1-5_2*NCC/3=O]/1/](data:image/png;base64,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)
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| 41 | dbPTM | 55824753 | |
| 45 | O-GlycoProteomDB | ||
| 52 | O-GlycoProteomDB | ||
| 52 | dbPTM | 55824759 | |
| 52 | PhosphoSitePlus |
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|
| 53 | O-GlycoProteomDB | ||
| 53 | dbPTM | 55824763 | |
| 53 | PhosphoSitePlus |
|
|
| 55 | O-GlycoProteomDB | ||
| 113 | PolarProtDb | 31844046 | |
| 113 | N-GlycoSiteAtlas | 24324730 , 25285165 | |
| 115 | PolarProtDb |
![WURCS=2.0/3,3,2/[AUd21122h_5*NCC/3=O][uxxxxh_2*NCC/3=O][uxxxxh]/1-2-3/a?|b?|c?}-{a?|b?|c?_a?|b?|c?}-{a?|b?|c?](data:image/png;base64,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)
|
27695962 |
| 117 | O-GlycoProteomDB | ||
| 119 | N-GlycoSiteAtlas | 25628020 | |
| 121 | O-GlycoProteomDB | ||
| 169 | O-GlycoProteomDB | ||
| 169 | dbPTM | 55830075 | |
| 169 | PhosphoSitePlus |
|
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | EGF-like_dom |
Interactions
| With (links to EBI) | #Exp | PubMed links |
|---|---|---|
| CRTP1_HUMAN | 3 | 32296183 |
| KR108_HUMAN | 3 | 32296183 |
Protein can be found in apical membrane,
Protein can be found in basal membrane,
Protein can be found both membranes,
Localisation is unknown.