PolarProtDb

Gene name

APLP2 (APPL2)

Experiment(s)

For this entry:

APLP2_HUMAN (APLP2)

Species	Homo sapiens
Localization	Basolateral plasma membrane
Reference	Touzelet O,Broadbent L,Armstrong SD,Aljabr W,Cloutman-Green E,Power UF,Hiscox JA (2020) The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins., Mol Cell Proteomics, 19, 793-807. [PubMed] [DOI]
Evidence level	Large-scale proteomics
Organ	Lung
Cell	Airway epithel cells
Notes	Only mock-transfected cells were considered

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	31	Signal peptide
32	693	Extracellular
694	716	Membrane
717	763	Cytoplasmic

Phosphorylation

Position	Resource
211	40721225
213	18908709
233	56657034
303	18006714
311	41660001
316	41660000
327	55374407
723	11500600
736	451599
750	454940
754	15228061
755	454253
757	455148

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
43	UniCarbKB	$WURCS=2.0/2,2,1/[uxxxxh_2*NCC/3=O][uxxxxh]/1-2/a?\|b?}-{a?\|b?$	30459171
43	O-GlycoProteomDB
242	O-GlycoProteomDB
289	O-GlycoProteomDB
296	O-GlycoProteomDB
303	O-GlycoProteomDB
367	PhosphoSitePlus
367	UniCarbKB	$WURCS=2.0/2,2,1/[uxxxxh_2*NCC/3=O][uxxxxh]/1-2/a?\|b?}-{a?\|b?$	30459171
367	dbPTM		55834349
367	O-GlycoProteomDB
371	PhosphoSitePlus
371	UniCarbKB	$WURCS=2.0/2,2,1/[uxxxxh_2*NCC/3=O][uxxxxh]/1-2/a?\|b?}-{a?\|b?$	30459171
371	dbPTM		55834355
371	O-GlycoProteomDB
380	PhosphoSitePlus
380	PolarProtDb		29296958
380	dbPTM		55834361
380	O-GlycoProteomDB
381	PhosphoSitePlus
381	O-GlycoProteomDB
381	dbPTM		55834367
481	O-GlycoProteomDB
541	Glyconnect	$WURCS=2.0/3,12,11/[axxxxm-1x_1-5][axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-1-2-2-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?$	29741879
541	Glyconnect	$WURCS=2.0/4,12,11/[Aad21122h-2x_2-6_5NCC/3=O][axxxxm-1x_1-5][axxxxh-1x_1-5_2NCC/3=O][axxxxh-1x_1-5]/1-2-2-3-3-3-3-4-4-4-4-4/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?$	29741879
541	Glyconnect	$WURCS=2.0/3,13,12/[axxxxm-1x_1-5][axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-1-1-2-2-2-2-3-3-3-3-3/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?\|l?\|m?$	29741879
541	Glyconnect	$WURCS=2.0/4,11,10/[Aad21122h-2x_2-6_5NCC/3=O][axxxxm-1x_1-5][axxxxh-1x_1-5_2NCC/3=O][axxxxh-1x_1-5]/1-2-3-3-3-3-4-4-4-4-4/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?\|j?\|k?$	29741879
541	N-GlycoSiteAtlas		19196183 , 25247386
541	PolarProtDb		31844046
541	UniPep
658	PhosphoSitePlus
658	dbPTM		55828131
658	O-GlycoProteomDB

Family and domain data

Database	Domain/Family name
InterPro	Amyloid_Cu-bd_sf
InterPro	Amyloid_glyco
InterPro	Amyloid_glyco_Cu-bd
InterPro	Amyloid_glyco_E2_domain
InterPro	Amyloid_glyco_extra
InterPro	Amyloid_glyco_heparin-bd
InterPro	Amyloid_glyco_heparin-bd_sf
InterPro	Amyloid_glyco_intracell_CS
InterPro	APP_amyloid_C
InterPro	APP_CUBD_CS
InterPro	E2_sf
InterPro	Kunitz_BPTI
InterPro	Kunitz_BPTI_sf
InterPro	PH-like_dom_sf
InterPro	Prtase_inh_Kunz-CS
Pfam	APP_amyloid
Pfam	APP_Cu_bd
Pfam	APP_E2
Pfam	APP_N
Pfam	Kunitz_BPTI

Interactions

With (links to EBI)	#Exp	PubMed links
JUN_HUMAN	3	20936779 , 20195357
A4_HUMAN	2	16193067
ETS1_HUMAN	2	20195357
APLP2_HUMAN	3	16193067
APLP1_HUMAN	2	16193067
DEDD_HUMAN	3	20195357
ESR1_HUMAN	2	31527615

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.