Gene name
CHRM3 (Chrm-3)
Experiment(s)
For this entry:
ACM3_HUMAN (CHRM3)
| Species | Homo sapiens |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Iverson HA,Fox D 3rd,Nadler LS,Klevit RE,Nathanson NM (2005) Identification and structural determination of the M(3) muscarinic acetylcholine receptor basolateral sorting signal., J Biol Chem, 280, 24568-75. [PubMed] [DOI] |
| Evidence level | Antibody staining + sub-protein manipulation |
| Organ | Kidney |
| Cell | MDCK cells (distal tubule) |
| Notes | A single dileucine-type motif was responsible for basolateral targeting |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 67 | Extracellular |
| 68 | 91 | Membrane |
| 92 | 106 | Cytoplasmic |
| 107 | 130 | Membrane |
| 131 | 139 | Extracellular |
| 140 | 163 | Membrane |
| 164 | 186 | Cytoplasmic |
| 187 | 207 | Membrane |
| 208 | 228 | Extracellular |
| 229 | 250 | Membrane |
| 251 | 493 | Cytoplasmic |
| 494 | 517 | Membrane |
| 518 | 524 | Extracellular |
| 525 | 546 | Membrane |
| 547 | 590 | Cytoplasmic |
Motifs gathered by PolarProtDb Team
| Start | End | Localisation | Type | PubMed |
|---|---|---|---|---|
| 275 | 281 | Basolateral plasma membrane | Dileucine-like |
Iverson HA,Fox D 3rd,Nadler LS,Klevit RE,Nathanson NM (2005) Identification and structural determination of the M(3) muscarinic acetylcholine receptor basolateral sorting signal., J Biol Chem, 280, 24568-75. [PubMed][DOI] |
Phosphorylation
Glycosylation
| Position | Site resource | Glycan resource | PubMed link(s) |
|---|---|---|---|
| 25 | O-GlycoProteomDB | ||
| 33 | O-GlycoProteomDB | ||
| 35 | O-GlycoProteomDB |
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | GPCR_Rhodpsn |
| InterPro | GPCR_Rhodpsn_7TM |
| InterPro | Musac_Ach_M3_rcpt |
| InterPro | Musac_Ach_rcpt |
| Pfam | 7tm_1 |
Interactions
| With (links to EBI) | #Exp | PubMed links |
|---|---|---|
| ACM5_HUMAN | 8 | 20398705 |
Protein can be found in apical membrane,
Protein can be found in basal membrane,
Protein can be found both membranes,
Localisation is unknown.