Gene name
Slc12a3 (Ncc,Tsc)
Experiment(s)
For this entry:
S12A3_MOUSE (Slc12a3)
| Species | Mus musculus |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Verlander JW,Miller RT,Frank AE,Royaux IE,Kim YH,Weiner ID (2003) Localization of the ammonium transporter proteins RhBG and RhCG in mouse kidney., Am J Physiol Renal Physiol, 284, F323-37. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Kidney |
| Cell | Distal tubules |
| Notes |
S12A3_MOUSE (Slc12a3)
| Species | Mus musculus |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Voets T,Nilius B,Hoefs S,van der Kemp AW,Droogmans G,Bindels RJ,Hoenderop JG (2004) TRPM6 forms the Mg2+ influx channel involved in intestinal and renal Mg2+ absorption., J Biol Chem, 279, 19-25. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Kidney |
| Cell | Distal tubules |
| Notes |
S12A3_MOUSE (Slc12a3)
| Species | Mus musculus |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Tokonami N,Takata T,Beyeler J,Ehrbar I,Yoshifuji A,Christensen EI,Loffing J,Devuyst O,Olinger EG (2018) Uromodulin is expressed in the distal convoluted tubule, where it is critical for regulation of the sodium chloride cotransporter NCC., Kidney Int, 94, 701-715. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Kidney |
| Cell | Distal tubules |
| Notes |
S12A3_MOUSE (Slc12a3)
| Species | Mus musculus |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Gotoh Y,Kita S,Fujii M,Tagashira H,Horie I,Arai Y,Uchida S,Iwamoto T (2015) Genetic knockout and pharmacologic inhibition of NCX2 cause natriuresis and hypercalciuria., Biochem Biophys Res Commun, 456, 670-5. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Kidney |
| Cell | Distal tubules |
| Notes |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 138 | Cytoplasmic |
| 139 | 157 | Membrane |
| 158 | 165 | Extracellular |
| 166 | 191 | Membrane |
| 192 | 212 | Cytoplasmic |
| 213 | 234 | Membrane |
| 235 | 257 | Extracellular |
| 258 | 277 | Membrane |
| 278 | 282 | Cytoplasmic |
| 283 | 304 | Membrane |
| 305 | 334 | Extracellular |
| 335 | 356 | Membrane |
| 357 | 372 | Cytoplasmic |
| 373 | 395 | Membrane |
| 396 | 449 | Extracellular |
| 450 | 473 | Membrane |
| 474 | 503 | Cytoplasmic |
| 504 | 523 | Membrane |
| 524 | 525 | Extracellular |
| 526 | 548 | Membrane |
| 549 | 564 | Cytoplasmic |
| 565 | 582 | Membrane |
| 583 | 584 | Extracellular |
| 585 | 603 | Membrane |
| 604 | 1002 | Cytoplasmic |
Phosphorylation
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | AA-permease/SLC12A_dom |
| InterPro | AA_permease_N |
| InterPro | SLC12_C |
| InterPro | SLC12A3 |
| InterPro | SLC12A_fam |
| Pfam | AA_permease |
| Pfam | AA_permease_N |
| Pfam | SLC12 |