Gene name
SLC12A2 (Nkcc1)
Experiment(s)
For this entry:
S12A2_MOUSE (SLC12A2)
| Species | Mus musculus |
|---|---|
| Localization | Apical plasma membrane |
| Reference |
Gregoriades JMC,Madaris A,Alvarez FJ,Alvarez-Leefmans FJ (2019) Genetic and pharmacological inactivation of apical Na<sup>+</sup>-K<sup>+</sup>-2Cl<sup>-</sup> cotransporter 1 in choroid plexus epithelial cells reveals the physiological function of the cotransporter., Am J Physiol Cell Physiol, 316, C525-C544. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Choroid plexus |
| Cell | Ependymal cells |
| Notes |
S12A2_MOUSE (SLC12A2)
| Species | Mus musculus |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Peña-Münzenmayer G,Catalán M,Cornejo I,Figueroa CD,Melvin JE,Niemeyer MI,Cid LP,Sepúlveda FV (2005) Basolateral localization of native ClC-2 chloride channels in absorptive intestinal epithelial cells and basolateral sorting encoded by a CBS-2 domain di-leucine motif., J Cell Sci, 118, 4243-52. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Large intestine |
| Cell | Crypt gland cells |
| Notes |
S12A2_MOUSE (SLC12A2)
| Species | Mus musculus |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Peña-Münzenmayer G,Catalán M,Cornejo I,Figueroa CD,Melvin JE,Niemeyer MI,Cid LP,Sepúlveda FV (2005) Basolateral localization of native ClC-2 chloride channels in absorptive intestinal epithelial cells and basolateral sorting encoded by a CBS-2 domain di-leucine motif., J Cell Sci, 118, 4243-52. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Small intestine |
| Cell | Crypt gland cells |
| Notes |
S12A2_MOUSE (SLC12A2)
| Species | Mus musculus |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Nejsum LN,Praetorius J,Nielsen S (2005) NKCC1 and NHE1 are abundantly expressed in the basolateral plasma membrane of secretory coil cells in rat, mouse, and human sweat glands., Am J Physiol Cell Physiol, 289, C333-40. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Sweat glands |
| Cell | Secretory cells |
| Notes | Immunogold labelling under electron microscope |
S12A2_MOUSE (SLC12A2)
| Species | Mus musculus |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Koumangoye R,Omer S,Delpire E (2019) A dileucine motif in the COOH-terminal domain of NKCC1 targets the cotransporter to the plasma membrane., Am J Physiol Cell Physiol, 316, C545-C558. [PubMed] [DOI] |
| Evidence level | Fluorescent tagging |
| Organ | Kidney |
| Cell | MDCK cells (distal tubule) |
| Notes | A dileucine-type motif (DLPPVLL) was proposed to mediate basolateral localization |
S12A2_MOUSE (SLC12A2)
| Species | Mus musculus |
|---|---|
| Localization | Basolateral plasma membrane |
| Reference |
Evans RL,Park K,Turner RJ,Watson GE,Nguyen HV,Dennett MR,Hand AR,Flagella M,Shull GE,Melvin JE (2000) Severe impairment of salivation in Na+/K+/2Cl- cotransporter (NKCC1)-deficient mice., J Biol Chem, 275, 26720-6. [PubMed] [DOI] |
| Evidence level | Antibody staining |
| Organ | Salivary gland |
| Cell | Acinar cells |
| Notes |
For ortholog entries:
Alignment and sequence features
Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).
CCTOP topology prediction
| Start | End | Localisation |
|---|---|---|
| 1 | 283 | Cytoplasmic |
| 284 | 305 | Membrane |
| 306 | 307 | Extracellular |
| 308 | 335 | Membrane |
| 336 | 356 | Cytoplasmic |
| 357 | 378 | Membrane |
| 379 | 401 | Extracellular |
| 402 | 420 | Membrane |
| 421 | 428 | Cytoplasmic |
| 429 | 448 | Membrane |
| 449 | 478 | Extracellular |
| 479 | 498 | Membrane |
| 499 | 514 | Cytoplasmic |
| 515 | 536 | Membrane |
| 537 | 594 | Extracellular |
| 595 | 616 | Membrane |
| 617 | 646 | Cytoplasmic |
| 647 | 668 | Membrane |
| 669 | 670 | Extracellular |
| 671 | 692 | Membrane |
| 693 | 708 | Cytoplasmic |
| 709 | 736 | Membrane |
| 737 | 1205 | Extracellular |
Phosphorylation
Glycosylation
Family and domain data
| Database | Domain/Family name |
|---|---|
| InterPro | AA-permease/SLC12A_dom |
| InterPro | AA_permease_N |
| InterPro | NKCC1 |
| InterPro | SLC12_C |
| InterPro | SLC12A1/SLC12A2 |
| InterPro | SLC12A_fam |
| Pfam | AA_permease |
| Pfam | AA_permease_N |
| Pfam | SLC12 |
Interactions
| With (links to EBI) | #Exp | PubMed links |
|---|---|---|
| STK39_MOUSE | 3 | 14563843 , 12386165 |
| MK14_MOUSE | 2 | 14563843 |
Protein can be found in apical membrane,
Protein can be found in basal membrane,
Protein can be found both membranes,
Localisation is unknown.