PolarProtDb

Gene name

MME (EPN)

Experiment(s)

For this entry:

NEP_HUMAN (MME)

Species	Homo sapiens
Localization	Apical plasma membrane
Reference	Paulusma CC,Groen A,Kunne C,Ho-Mok KS,Spijkerboer AL,Rudi de Waart D,Hoek FJ,Vreeling H,Hoeben KA,van Marle J,Pawlikowska L,Bull LN,Hofmann AF,Knisely AS,Oude Elferink RP (2006) Atp8b1 deficiency in mice reduces resistance of the canalicular membrane to hydrophobic bile salts and impairs bile salt transport., Hepatology, 44, 195-204. [PubMed] [DOI]
Evidence level	Antibody staining
Organ	Liver
Cell	Hepatocytes
Notes	Apical localization depends on ATP8B1

For ortholog entries:

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	26	Cytoplasmic
27	42	Membrane
43	750	Extracellular

Phosphorylation

Position	Resource
4	4729965
6	3850156
11	14576231
15	15421454
207	50775345
387	460616
482	3535057
497	3535062
502	3535067
507	55370007
713	55370011

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
145	Glyconnect	$WURCS=2.0/2,9,8/[axxxxh-1x_1-5_2*NCC/3=O][axxxxh-1x_1-5]/1-1-1-1-2-2-2-2-2/a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?_a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?}-{a?\|b?\|c?\|d?\|e?\|f?\|g?\|h?\|i?$	29741879
145	dbPTM		10669592 , 12754519 , 14747736 , 17704566
145	N-GlycoSiteAtlas		24741114 , 24495048
145	PolarProtDb		31844046
145	UniPep
145	UniProtKB		10669592 , 12754519 , 14747736 , 17704566
145	PDB		32071687
285	PolarProtDb		31844046
285	UniProtKB		12754519
285	UniPep
285	N-GlycoSiteAtlas		24495048
285	dbPTM		12754519
285	PDB		32071687
311	N-GlycoSiteAtlas		24495048
325	PDB		32071687
325	dbPTM		10669592 , 14747736 , 17704566
325	N-GlycoSiteAtlas		24495048
325	UniProtKB		10669592 , 14747736 , 17704566
628	PDB		32071687
628	dbPTM		10669592 , 14747736 , 17704566 , 22766194
628	UniProtKB		10669592 , 14747736 , 17704566 , 22766194

Family and domain data

Database	Domain/Family name
InterPro	MetalloPept_cat_dom_sf
InterPro	MME/CD10/NEP
InterPro	Peptidase_M13
InterPro	Peptidase_M13_C
InterPro	Peptidase_M13_dom_2
InterPro	Peptidase_M13_N
Pfam	Peptidase_M13
Pfam	Peptidase_M13_N

Interactions

With (links to EBI)	#Exp	PubMed links
HSPB1_HUMAN	4	17342744
HS71B_HUMAN	3	17342744

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.