PolarProtDb

Gene name

IL6 (IFNB2)

Experiment(s)

For this entry:

IL6_HUMAN (IL6)

Species	Homo sapiens
Localization	Apical plasma membrane
Reference	Polet M,Laloux L,Cambier S,Ziebel J,Gutleb AC,Schneider YJ (2020) Soluble silver ions from silver nanoparticles induce a polarised secretion of interleukin-8 in differentiated Caco-2 cells., Toxicol Lett, 325, 14-24. [PubMed] [DOI]
Evidence level	Selective medium analysis
Organ	Large intestine
Cell	CACO-2 cells (enterocytes)
Notes

IL6_HUMAN (IL6)

Species	Homo sapiens
Localization	Apical plasma membrane
Reference	Chow AW,Liang JF,Wong JS,Fu Y,Tang NL,Ko WH (2010) Polarized secretion of interleukin (IL)-6 and IL-8 by human airway epithelia 16HBE14o- cells in response to cationic polypeptide challenge., PLoS One, 5, e12091. [PubMed] [DOI]
Evidence level	Selective medium analysis
Organ	Lung
Cell	16HBE14o- cells (airway epithel cells)
Notes

For ortholog entries:

IL6_BOVIN

Alignment and sequence features

Wheel: zoom in/out; drag: scroll left/right. Sequences and features are drawn in three lines. Bottom line: topology (red bar: cytoplasmic region; yellow bar: transmembrane region; transmembrane re-entrant loop; blue bar: extracellular region; black bar: signal peptide). Middle line: amino acid sequence (grey bar or one letter code). Top line: post translational modifications (Y: N-glycosylation site; Y: O-glycosylation site;P: phosphorylation site), intrinsically disordered regions (light green bar: IDR) and short linear motifs (light steel blue bar: ELM region).

CCTOP topology prediction

Start	End	Localisation
1	212	Extracellular

Phosphorylation

Position	Resource
135	23115604
136	23115606
165	23115608

Glycosylation

Position	Site resource	Glycan resource	PubMed link(s)
73	dbPTM		1883960
73	N-GlycoSiteAtlas		25628020
73	OGlycoBase		94266765
73	UniProtKB		1883960
160	PolarProtDb	$WURCS=2.0/3,3,2/[AUd21122h_5NCC/3=O][uxxxxh_2NCC/3=O][uxxxxh]/1-2-3/a?\|b?\|c?}-{a?\|b?\|c?_a?\|b?\|c?}-{a?\|b?\|c?$	27695962
166	dbPTM		8206884
166	OGlycoBase		94266765
170	dbPTM		14970177
171	dbPTM		14970177

Family and domain data

Database	Domain/Family name
InterPro	4_helix_cytokine-like_core
InterPro	IL-6
InterPro	IL-6/GCSF/MGF
InterPro	IL6/GCSF/MGF_CS
Pfam	IL6

Interactions

With (links to EBI)	#Exp	PubMed links
SORT_HUMAN	4	25401472
IL6RB_HUMAN	2	18775332 , 12829785
IL6RA_HUMAN	3	18775332 , 12829785 , 28265003
SORL_HUMAN	4	28265003
ZBT16_HUMAN	2	25241761 , 16169070

Protein can be found in apical membrane, Protein can be found in basal membrane, Protein can be found both membranes, Localisation is unknown.